不同剂量红光对Ⅰ型糖尿病大鼠背部急性创面的疗效研究  

Study on the Effect of Different Doses of Red Light on Acute Back Wounds in TypeⅠDiabetic Rats

作　　者：杨伟江 张文涛 罗杰夫 岳陈达 岑蕊言 雷霞 Yang Weijiang;Zhang Wentao;Luo Jiefu;Yue Chenda;Cen Ruiyan;Lei Xia(Department of Dermatology,Duping Hospital Affiliated to the Third Military Medical University,Chongqing 400042,China)

机构地区：[1]陆军军医大学大坪医院皮肤科,重庆400042

出　　处：《应用激光》2021年第6期1346-1352,共7页Applied Laser

基　　金：国家自然科学基金资助项目(81773348,81573071)。

摘　　要：为研究不同治疗剂量的红光对Ⅰ型糖尿病SD大鼠背部急性创面的愈合效果,对大鼠采取腹腔注射链脲佐菌素(60 mg/kg)溶液,2周后测外周血,血糖浓度≥16.7 mmol/L时认为Ⅰ型糖尿病模型造模成功。将60只糖尿病大鼠随机分成5组,分别为对照组(L0,不治疗)、治疗组一(L1,12 J/cm^(2))、治疗组二(L2,24 J/cm^(2))、治疗组三(L3,48 J/cm^(2))、治疗组四(L4,96 J/cm^(2))。用8 mm直径打孔器在大鼠背部中线两侧对称地制造2个急性创面。1 d后用633 nm±10 nm波长红光按各分组的不同能量密度照射创面。创面后第0、3、7、10天行创面拍照,第3、7、10天分别随机取4只大鼠行创面取材。石蜡包埋后做HE染色、Masson染色、CD31免疫组化学染色、CD68免疫荧光染色。试验结果显示:创面愈合率显示治疗组愈合加快,其中L3组作用效果显著;HE显示治疗组的创面再上皮化加快,第7天时L3促进作用明显;CD31免疫组织化学染色显示治疗组能促进创面下血管生成,L3和L4最为突出;CD68免疫荧光显示治疗组能减弱第10天时创面炎症浸润程度,其中L2、L3阳性率最低。试验总结:在试验的能量密度范围内,红光能促进Ⅰ型糖尿病SD大鼠背部创面愈合,且当能量密度为48 J/cm^(2)时(L3),其促进效果最为显著。To study the healing effect of different therapeutic doses of red light on acute wounds of typeⅠdiabetes SD rats.2 weeks after intraperitoneal injection of Streptozocin(60 mg/kg)solution,rats that peripheral blood sugar over 16.7 mmol/L were considered of diabetes models.Sixty diabetic rats were randomly divided into 5 groups:control group(L0,no treatment),treatment group 1(L1,12 J/cm^(2)),treatment group 2(L2,24 J/cm^(2)),treatment group 3(L3,48 J/cm^(2)),and treatment group 4(L4,96 J/cm^(2)).Two acute wounds were made symmetrically on both sides of the midline of the rat back with a hole punch of 8 mm diameter.One day later,wound was irradiated with red light of wavelength of 633 nm±10 nm at different energy densities of each group.The wounds were photographed on day 0,3,7 and 10 after punching.On day 3,7 and 10,4 rats were randomly selected for wound sampling.After paraffin embedding,HE staining,Masson staining,CD31 immunohistochemical staining and CD68 immunofluorescence staining were performed.Results:The wound healing rate showed that red light treatment can accelerate wound healing,and the effect of L3 group was most significant.HE showed that re-epithelialization of wounds in the treatment groups were accelerated and L3 was the best at day 7.CD31 immunohistochemical staining showed that treatment groups could promote angiogenesis and L3 and L4 were the most prominent.CD68 immunofluorescence showed that treatment groups could reduce the degree of wound inflammatory infiltration at the 10 th day,and the positive rates of L2 and L3 were the lowest.Conclusion:Within the scope of the energy density of the experiment,red light can promote typeⅠdiabetes SD rats back wound healing,and the most notable promoting effect energy density is 48 J/cm^(2).

关 键 词：红光 大鼠 糖尿病 创面愈合 

分 类 号：R318.51[医药卫生—生物医学工程] R312[医药卫生—基础医学]