乌苏里貉GHR基因第10外显子SNP分析  

Analysis on SNP of GHR exon 10 fragment in Wusuli raccoon dog

作　　者：荣敏[1] 李伟[2] 李鑫 任二军[2] 刘洁[2] 邢秀梅[1] RONG Min;LI Wei;LI Xin;REN Erjun;LIU Jie;XING Xiumei(State Key Laboratory of Special Economic Animal Molecular Biology,Insistule of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences,Changchun 130112,China;Technological Innovation Center for Fur Animal Breeding of Hebei,Shijiazhuang Academy of Agricultural and Forestry Sciences,Shijiazhuang 050041,China)

机构地区：[1]中国农业科学院特产研究所特种经济动物分子生物学重点实验室,长春130112 [2]石家庄市农林科学研究院河北省毛皮动物养殖技术创新中心,石家庄050041

出　　处：《黑龙江畜牧兽医》2022年第1期126-129,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：河北省重点研发项目(19226344D);中国农业科学院科技创新工程-特种动物遗传资源科技创新团队项目(CAAS-ASTIP-201X-ISAPS)。

摘　　要：为了研究乌苏里貉资源群体中GHR基因的单核苷酸多态性(SNP),试验选取生长性状差异较大的两个乌苏里貉群体分别构建DNA池,对GHR基因的第10外显子进行PCR扩增并测序。结果表明:发现5个SNP位点,分别为T911C、C1112T、G1454A、G1522A、C2279T。其中T911C为错义突变,可导致苯丙氨酸改变为亮氨酸;C1112T为错义突变,可导致丝氨酸改变为苯丙氨酸;G1454A为同义突变,氨基酸不发生改变;G1522A为错义突变,可导致精氨酸改变为赖氨酸;C2279T为错义突变,可导致丝氨酸改变为亮氨酸。表明这4个错义突变的SNP位点有可能与乌苏里貉生长性状相关。In order to study the single nucleotide polymorphisms(SNP) of GHR gene in Wusuli raccoon dogs resource population, two Wusuli raccoon dog groups with significant differences in growth traits were selected to construct DNA pools respectively. The exon 10 of GHR gene was amplified by PCR and sequenced. The results showed that five SNPs were found, which were T911 C, C1112 T, G1454 A, G1522 A and C2279 T. Among them, T911 C is a missense mutation, which can cause a change from phenylalanine to leucine. C1112 T is a missense mutation that can change serine to phenylalanine. G1454 A is the synonymous mutation and amino acid does not change. G1522 A is a missense mutation that can change arginine to lysine. C2279 T is a missense mutation that can change serine to leucine. It indicated that the SNP loci of these four missense mutations might be related to the growth traits of Wusuli raccoon dog.

关 键 词：乌苏里貉 GHR基因 exon10 SNP 多态性 

分 类 号：S865.24[农业科学—野生动物驯养]