艾灸对佐剂性关节炎大鼠膝关节滑膜组织中调控铁死亡相关因子的影响  

Effects of moxibustion on p53,SLC7A11,and GPX4 expression in synovial tissues of rats with adjuvant arthritis

作　　者：彭传玉[1] 胡玲 吴子建 王洁 蔡荣林[1] PENG Chuan-yu;HU Ling;WU Zi-jian;WANG Jie;CAI Rong-lin(College of Acupuncture-moxibustion and Tuina,Anhui University of Chinese Medicine,Hefei 230038,China;Institute of Acupuncture and Meridian,Anhui Academy of Chinese Medicine,Hefei 230038,China)

机构地区：[1]安徽中医药大学针灸推拿学院,合肥230038 [2]安徽省中医药科学院针灸经络研究所,合肥230038

出　　处：《针刺研究》2022年第1期21-26,共6页Acupuncture Research

基　　金：安徽高校自然科学研究重点项目(No.KJ2018A0296、KJ2017A300);国家重点基础研究发展计划项目(No.2015CB554504)。

摘　　要：目的:观察艾灸对佐剂性关节炎(AA)大鼠滑膜组织p53、胱氨酸/谷氨酸逆向转运蛋白溶质载体家族7成员11(SLC7A11)、谷胱甘肽过氧化物酶4(GPX4)表达的影响,探讨艾灸治疗AA的作用机制。方法:SD大鼠随机分为正常组、模型组、艾灸组和药物组,每组20只。采用风寒湿环境因素结合完全弗氏佐剂建立AA大鼠模型。艾灸组予艾条悬灸"肾俞""足三里",两穴交替使用,药物组给予雷公藤多苷片混悬液(8 mg/kg)灌胃,均每日1次,连续15 d。采用HE染色法观察大鼠右膝关节滑膜组织病理形态学变化;Western blot法和荧光定量PCR法检测大鼠右膝关节滑膜组织p53、SLC7A11、GPX4蛋白和mRNA的表达;比色法和荧光探针法检测大鼠血清谷胱甘肽(GSH)含量和活性氧(ROS)的水平。结果:与正常组比较,模型组大鼠右膝关节滑膜异常增生,大量炎性细胞浸润;滑膜组织p53蛋白和mRNA表达、血清ROS表达强度明显升高(P<0.01),滑膜组织SLC7A11、GPX4蛋白和mRNA表达、血清GSH水平显著降低(P<0.01)。与模型组比较,艾灸组和药物组滑膜组织损伤明显改善,滑膜组织p53蛋白和mRNA表达、血清ROS表达强度显著降低(P<0.01),滑膜组织SLC7A11、GPX4蛋白和mRNA表达、血清GSH水平明显升高(P<0.01)。与艾灸组比较,药物组滑膜组织病理形态学无明显差异,滑膜组织p53蛋白表达、血清ROS水平明显升高(P<0.05),GPX4蛋白表达、血清GSH水平显著降低(P<0.05)。结论:艾灸改善AA模型大鼠膝关节滑膜组织炎性反应的作用可能与其调控铁死亡相关因子的表达密切相关。Objective To observe the effects of moxibustion on p53,cystine/glutamate antiporter solute carrier family 7 member 11(SLC7 A11),and glutathione peroxidase 4(GPX4)in synovial tissues of rats with adjuvant arthritis(AA),so as to explore the mechanism of moxibustion in alleviating rheumatoid arthritis.Methods Eighty rats were randomly divided into normal,model,moxibustion,and medication groups(n=20 in each group).The AA model was established by exposure to wind,cold,and damp environmental factors combined with injection of complete Freund’s adjuvant.Rats in the moxibustion group received suspended moxibustion at“Shenshu”(BL23)and“Zusanli”(ST36)alternately,while those in the medication group were treated with tripterygium glycoside tablet suspension(8 mg/kg)by gavage,once a day,for 15 successive days.The pathological change in synovial tissue of rat right knee joint was observed by HE staining.The protein and mRNA expression levels of p53,SLC7 A11,and GPX4 in the synovial tissue were detected by Western blot and quantitative real-time PCR,respectively.The se-rum glutathione(GSH)and reactive oxygen species(ROS)contents were measured by colorimetry and fluorescence probe me-thod.Results Compared with the normal group,the model group exhibited synovial hyperplasia of the right knee joint,massive inflammatory cell infiltration,up-regulated mRNA and protein expression of p53 in synovial tissue,elevated serum ROS content(P<0.01),down-regulated mRNA and protein expression of SLC7 A11 and GPX4 in synovial tissue,and lowered serum GSH content(P<0.01).Comparison with the model group showed that the synovial injuries in the moxibustion and medication groups were obviously alleviated.The mRNA and protein expression levels of p53 in the synovial tissues and the serum ROS content declined significantly(P<0.01),while the mRNA and protein expression of SLC7 A11 and GPX4 in the synovial tissues and the se-rum GSH content increased(P<0.01).There was no significant difference in histopathological change of synovial tissue bet

关 键 词：艾灸 佐剂性关节炎 铁死亡 滑膜 炎性反应 

分 类 号：R245.81[医药卫生—针灸推拿学]