聚苯乙烯微塑料与铜离子单一和复合污染对THP-1细胞的DNA损伤效应研究  

Studying on DNA damage effects of single and combined contamination of copper ion and polystyrene microplastics on THP-1 cells

作　　者：尹艳 王海燕 韩大雄[2] 林彩 林锡煌 单柏 YIN Yan;WANG Haiyan;HAN Daxiong;LIN Cai;LIN Xihuang;SHAN Bo(Third Institute of Oceanography, MNR, Xiamen 361005, China;School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, China)

机构地区：[1]自然资源部第三海洋研究所,福建厦门361005 [2]厦门大学药学院,福建厦门361102

出　　处：《应用海洋学学报》2022年第1期86-93,共8页Journal of Applied Oceanography

基　　金：厦门海洋研究开发院资助项目(2018010);自然资源部第三海洋研究所基本科研业务费资助项目(HE01-190702(6))。

摘　　要：采用单细胞凝胶电泳实验研究了不同浓度的Cu^(2+)(10、50、100、500、1000、2500、5000μg/L)和不同粒径(0.1、0.2、0.3、0.5、0.7、1.0、2.0μm)、不同浓度(1、10、15、20、25 mg/mL)的聚苯乙烯微塑料对人单核细胞白血病(Human Acute Monocytic Leukemia,THP-1)细胞的DNA损伤效应,结果表明:单一污染时,Cu^(2+)(≥50μg/L)对THP-1细胞的DNA损伤极显著(p<0.01)。而且,DNA损伤值随Cu^(2+)浓度的增大而增加。2.0μm粒径的聚苯乙烯微塑料(10 mg/mL)对THP-1细胞产生明显的DNA损伤效应。复合污染作用下对THP-1细胞的DNA损伤效应,相比单一污染明显增强,而且DNA损伤值比单一污染的理论加和值高。本研究表明Cu^(2+)和聚苯乙烯微塑料均对DNA产生损伤效应,显示一定的基因毒性,二者复合对损伤具有交互增强的作用。The effects of different concentrations of Cu^(2+)(10,50,100,500,1000,2500,5000μg/L)and particle sizes(0.1,0.2,0.3,0.5,0.7,1.0,2.0μm)of polystyrene microplastics on DNA damage in human acute monocytic leukemia(THP-1 cells)were investigated by single-cell gel electrophoresis experiments.As results,≥50μg/L Cu^(2+)caused extremely significant DNA damage to THP-1 cells(p<0.01).Moreover,the extent of DNA damage in THP-1 cells increased with increasing Cu^(2+)concentration.In the other hand,2.0-μm particle size polystyrene microplastics(10 mg/mL)produced significant DNA damage in THP-1 cells.Under combined contamination,25 mg/mL microplastics(2.0μm)and Cu^(2+)(500μg/L)produced significant DNA damages in THP-1 cells,and the actual effects on DNA damage were much worse than the theoretical combination effects of single contamination.This study indicated that either Cu^(2+)and polystyrene microplastics caused damage effect on DNA,showing a certain amount of genotoxicity,and the combination of the two had interactive enhancement damage effect on THP-1 cells.

关 键 词：海洋环境科学 DNA损伤 单细胞凝胶电泳 Cu^(2+) 微塑料 聚苯乙烯 

分 类 号：P76[天文地球—海洋科学]