分子粪便学在野生动物食性研究中的应用  

Application of Molecular Scatology in the Study of Wild Animal Feeding Habits

作　　者：黄乃莹 刘艳华[1] HUANG Naiying;LIU Yanhua(College of Wildlife and Protected Area,Northeast Forestry University,Harbin,150040,China)

机构地区：[1]东北林业大学野生动物与自然保护地学院,哈尔滨150040

出　　处：《野生动物学报》2022年第1期266-269,共4页CHINESE JOURNAL OF WILDLIFE

基　　金：黑龙江省省属科研院所科研业务费项目(CZKYF2021B002)。

摘　　要：探讨分子粪便学在食性分析中的常用技术,概述了各项技术的概念、操作方法和最新进展。结果表明:分子粪便学方法在国外的动物食性研究中应用较多,与传统食性研究方法相比,利用植物DNA进行测序得到的结果更快速、更高效,并可降低动物消化率对结果的影响。建议研究者可以通过结合当地植被情况建立植物DNA条形码数据库对结果进行修正,对于近缘种植物可以采用特异性引物进行区分。This paper discusses the commonly used techniques of molecular scatology in food habit analysis and outlines the concepts,operation methods,and latest progress of each technique.The results show that molecular scatology methods are widely used in wild animal food habit research abroad;compared with traditional food habit analysis methods,the results obtained by sequencing plant DNA are faster and more efficient,and the effect of animal digestibility on the results can be reduced.It is suggested that researchers can revise the results by establishing a plant DNA barcode database in combination with local vegetation conditions,and specific primers can be used to distinguish closely related plants.

关 键 词：野生动物 分子粪便学 食性 

分 类 号：S953[农业科学—水产养殖]