Mirror-enhanced scanning light-field microscopy for long-term high-speed 3D imaging with isotropic resolution  

作　　者：Bo Xiong Tianyi Zhu Yuhan Xiang Xiaopeng Li Jinqiang Yu Zheng Jiang Yihan Niu Dong Jiang Xu Zhang Lu Fang Jiamin Wu Qionghai Dai 

机构地区：[1]Department of Automation,Tsinghua University,Beijing 100084,China [2]Institute for Brain and Cognitive Sciences,Tsinghua University,Beijing 100084,China [3]Beijing Laboratory of Brain and Cognitive Intelligence,Beijing Municipal Education Commission,Beijing 100084,China [4]State Key Laboratory of Membrane Biology,Tsinghua University-Peking University Joint Centre for Life Sciences,Beijing Frontier Research Center for Biological Structure,School of Life Sciences,Tsinghua University,Beijing 100084,China [5]Beijing Institute of Collaborative Innovation,Beijing 100094,China [6]Department of Electronic Engineering,Tsinghua University,Beijing 100084,China

出　　处：《Light(Science & Applications)》2021年第12期2369-2379,共11页光（科学与应用)（英文版）

摘　　要：Various biological behaviors can only be observed in 3D at high speed over the long term with low phototoxicity.Light-field microscopy(LFM)provides an elegant compact solution to record 3D information in a tomographic manner simultaneously,which can facilitate high photon efficiency.However,LFM still suffers from the missing-cone problem,leading to degraded axial resolution and ringing effects after deconvolution.Here,we propose a mirrorenhanced scanning LFM(MiSLFM)to achieve long-term high-speed 3D imaging at super-resolved axial resolution with a single objective,by fully exploiting the extended depth of field of LFM with a tilted mirror placed below samples.To establish the unique capabilities of MiSLFM,we performed extensive experiments,we observed various organelle interactions and intercellular interactions in different types of photosensitive cells under extremely low light conditions.Moreover,we demonstrated that superior axial resolution facilitates more robust blood cell tracking in zebrafish larvae at high speed.

关 键 词：RESOLUTION MIRROR FIELD 

分 类 号：TH742[机械工程—光学工程]