三种不同方法检测登革热临床诊断病例的比较研究  

Comparative study on three different detection methods for clinically diagnosed dengue cases

作　　者：秦圆方[1] 张欣[2] 张复春[3] 朱立国[1] Qin Yuanfang;Zhang Xin;Zhang Fuchun;Zhu Liguo(Acute infectious disease prevention and Control Institute,Jiangsu Provincial Center for Disease Control and Prevention,Nanjing 210009,China;Pathogenic Microorganism Laboratory,Guangdong Provincial Center for Disease Control and Prevention,Guangzhou 510440,China;Infectious Disease Department,the Eighth Affiliated Hospital of Guangzhou Medical University,Guangzhou 510180,China)

机构地区：[1]江苏省疾病预防控制中心急性传染病防制所,南京210009 [2]广东省疾病预防控制中心病原微生物检验所,广州510440 [3]广州医科大学附属市八医院感染科,510180

出　　处：《国际病毒学杂志》2021年第6期504-508,共5页International Journal of Virology

基　　金：江苏省社会发展项目(BE2021739);江苏省青年医学人才(QNRC2016546)。

摘　　要：目的探讨NS1抗原、IgM抗体及核酸等检测方法判定登革热临床诊断病例的效果,为防控疫情提供科学依据。方法收集426份登革热临床诊断病例血清样本,采用胶体金法检测登革病毒NS1抗原和IgM抗体,采用荧光RT-PCR法进行登革病毒核酸分型检测。结果NS1抗原检测与荧光RT-PCR检测结果配对卡方检验差异无统计学意义,但明显优于IgM抗体检测。两种NS1抗原之间,两种IgM抗体之间检测结果差异无统计学意义。2019年广东与江苏两地登革热疫情以1型登革病毒初次感染为主。结论三种方法的综合比较表明胶体金法NS1抗原检测检测结果与荧光RT-PCR差异无统计学意义,且简便经济,优于另外两种方法,更适用于登革热临床早期检测及大规模流行病学筛查。Objective To investigate capability of the detection methods for NS1 antigen,IgM antibody and nucleic acid to identify clinically diagnosed dengue cases,so as to provide scientific evidence for the control and prevention of the epidemics.Methods A total of 426 serum samples were collected from clinically diagnosed dengue patients.The samples were tested respectively for NS1 antigen and IgM antibody by colloidal gold methods.Fluorescent RT-PCR was used to detect types of dengue virus..Results There was no statistically significant difference in the results of NS1 colloidal gold method and the RT-qPCR tests by paired Chi-square test.NS1 tests were obviously better than IgM colloidal gold methods.No significant differences were found between the two NS1 methods or the two IgM colloidal gold methods.The primary infection by dengue virus serotype 1 was dominant during the dengue epidemic in Guangdong province and Jiangsu province in 2019.Conclusions The comparison of the three methods indicated that the results of NS1 colloidal gold method had no statistically significant difference from those by RT-qPCR,and were convenient and inexpensive.The method was better than the other two methods and suitable for early diagnosis of dengue fever and for large scale epidemiological screening.

关 键 词：登革热 NS1抗原 胶体金 荧光RT-PCR 临床诊断病例 

分 类 号：R512.8[医药卫生—内科学]