不同进样方式精子计数池和一次性计数池结构因素对精子活力的影响研究  

Influence of different filling method-related sperm counting chambers and structural factors of disposable chambers on sperm motility

作　　者：郑丽军 张静微 彭娅娅[1] 王皓莉 周小棉 王维[1] ZHENG Li-jun;ZHANG Jing-wei;PENG Ya-ya;WANG Hao-li;ZHOU Xiao-mian;WANG Wei(Center of Reproductive Medicine,Guangzhou First Peopled Hospital,Guangzhou,Guangdong 510180,China;Guangzhou Biotron of Research Institute Microfluidic Chip,Guangzhou,Guangdong 510180,China)

机构地区：[1]广州市第一人民医院生殖医学中心,广东广州510180 [2]广州市宝创微流控芯片研究院,广东广州510180

出　　处：《中华男科学杂志》2021年第11期995-1000,共6页National Journal of Andrology

摘　　要：目的:应用计算机辅助精液分析(CASA)系统评价不同进样方式精子计数池和一次性计数池结构因素对精子活力的影响。方法:用四种精子计数池:以液滴式进样为代表的Makler池、以毛细管虹吸进样为代表的Leja计数池和结构改变(磨边和盖玻片厚度)的Leja精子计数池,应用CASA系统检测76例门诊患者精液标本活力和动力学参数,并进行比较分析。结果:液滴式进样的Makler计数池所获得的前向运动精子百分率(PR%)、非前向运动精子百分率(NP%)、总活动率(PR+NP%)、精子平均曲线运动速度(VCL)、平均路径速度(VAP)显著高于以毛细管虹吸式进样的Leja计数池和结构改变计数池(P<0.05),其他精子参数,如平均直线运动速度(VSL)、精子平均鞭打频率(BCF)、运动的直线性(LIN)、运动的摆动性(WOB)及运动的前向性(STR)均无统计学意义上的差异;毛细管虹吸式进样(Leja计数池为代表)的精子浓度、前向运动精子百分率(PR)、非前向运动精子百分率(NP)和总活动率(PR+NP)等其他参数与结构改变式计数池相比无统计学差异(P>0.05);Leja计数池的盖玻片厚度和进样端盖玻片磨边对精子动力学参数的影响没有统计学差异(P>0.05)。结论:液滴式进样计数池所获得的精子活力和动力学参数显著高于毛细管虹吸式进样的Leja计数池所获得的值;Leja计数池的磨边与盖玻片厚度等结构因素对精子各参数分析不产生影响。Objective:To evaluate the effects of different filling method-related sperm counting chambers and the structural factors of Leja counting chambers on sperm motility using computer-assisted sperm analysis(CASA).Methods:Using drop-filled Makler,capillary-loaded Leja and structurally modified Leja sperm counting chambers,we measured sperm concentration,the percentages of progressively motile sperm(PMS)and non-progressively motile sperm(NPMS),total sperm motility,curvilinear velocity(VCL),average path velocity(VAP),straight line velocity(VSL),beat-cross frequency(BCF),linearity(LIN),wobble(WOB)and straightness(STR)in the semen samples of 76 males by CASA and compared them between different chambers.Results:The drop-filled Makler sperm counting chamber achieved remarkably higher PMS,NPMS,total sperm motility,VCL and VAP than the Leja chambers(P<0.05).There were no statistically significant differences in VSL,BCF,LIN,WOB and STR between the Makler and Leja chambers(P>0.05),or in sperm concentration,PMS,NPMS and total sperm motility between the capillary-loaded and structurally modified Leja counting chambers(P>0.05).The ground edge and thickness of the coverslip of the Leja counting chamber produced no significant inference on the kinetic sperm parameters(P>0.05).Conclusion:The drop-filled sperm counting chamber achieves significantly higher sperm motility and kinetic parameters than the capillary-loaded Leja chamber.The structural factors such as the ground edge and thickness of the coverslip of the Leja counting chamber do not influence the analysis of sperm parameters.

关 键 词：精子计数池 进样方式 盖玻片厚度 盖玻片磨边 精子相关参数 

分 类 号：R698.2[医药卫生—泌尿科学]