红参松花粉片对阿尔茨海默症大鼠空间学习记忆能力及海马神经细胞凋亡的影响  

Red ginseng pine pollens improve spatial learning and memory possibly by inhibition of hippocampal neuron apoptosis in a rat model of Alzheimer’s disease

作　　者：李学秀 崔嗣庚[2] 吕运一 LI Xuexiu;CUI Sigeng;Lü Yunyi(Department of Neurology,the Fourth Medical Center of PLA General Hospital,100049,Beijing,China;Yanbian Leafoo Life Science Institute,Yanji,133000,Yanji,Jilin,China;Department of Shenzhen Polytechnic,518055,Shenzhen,Guangdong,China)

机构地区：[1]解放军总医院第四医学中心,北京100049 [2]深圳职业技术学院医护学院,吉林延吉133000 [3]延边利孚生命科学研究院,广东深圳518055

出　　处：《北京师范大学学报（自然科学版）》2021年第6期854-858,共5页Journal of Beijing Normal University(Natural Science)

基　　金：颐瑙元研究资助项目(SZLF20190806)。

摘　　要：通过Aβ25-35联合D-半乳糖诱导,建立实验性大鼠阿尔茨海默症(Alzheimer’s disease,AD)模型,采用Morris水迷宫实验,评估红参松花粉片对AD模型大鼠学习记忆能力和海马神经细胞凋亡的影响.同时,运用Western blot、RT-qPCR及TUNEL法,检测AD模型大鼠海马神经细胞中BCL2、BAX、p-AKT及p-GSK3β的含量.研究表明:与模型组相比,红参松花粉片高剂量组能显著缩短模型大鼠逃脱潜伏期和游泳距离(P<0.05),显著增加搜索时间百分比(P<0.05);红参松花粉片高、低剂量给药组上调了AD模型大鼠海马神经细胞中Bcl-2、p-AKT及p-GSK3β的表达,下调了BAX的表达,显示AD模型大鼠海马神经细胞凋亡过程明显减缓,说明红参松花粉片能改善AD模型大鼠学习记忆的能力,对海马神经凋亡起到了一定的抑制作用.Changes in learning and memory and hippocampal neuron apoptosis after administration of red ginseng pine pollen tablets were investigated in a model of Alzheimer’s disease(AD)rat.Rats were randomly divided into 4 groups of 10 each.Alzheimer’s disease was induced after administration of Aβ25-35 plus D-galactose.Morris water maze test was used to evaluate any changes in learning and memory as well as hippocampal neuron apoptosis.Western blot,RT-qPCR and TUNEL assay were performed to detect content of BCL2,BAX,p-AKT and p-GSK3βin hippocampal neurons in AD model rats.Compared with the model controls,escape latency and swimming distance in the drugged model rats were significantly shortened(P<0.05),percentage of searching time was significantly increased(P<0.05),in high-dose group.BCL2,p-AKT and p-GSK3βin hippocampal neurons was up-regulated,but Bax was down-regulated after administration of both high-and low-doses of the pollen tablets.Meanwhile,hippocampal neuron apoptosis was significantly inhibited by pollen tablet administrations.These data suggest that pollen tablets of red ginseng pine improve learning and memory in AD rats,possibly by inhibition of hippocampal neuronal apoptosis.

关 键 词：红参 松花粉 阿尔茨海默症 凋亡 

分 类 号：Q2[生物学—细胞生物学]