有翅型和无翅型小麦禾谷缢管蚜转录组差异分析  

Analysis of transcriptomic differences between winged and wingless bird cherry-oat aphid Rhopalosiphum padi on wheat

作　　者：鲁艳辉[1] 虞飞博 郭嘉雯 田俊策[1] 郑许松[1] 吕仲贤[1] Lu Yanhui;Yu Feibo;Guo Jiawen;Tian Junce;Zheng Xusong;Lü Zhongxian(Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,Zhejiang Province,China;Department of Plant Protection,Yueqing Agricultural and Rural Development Center,Yueqing 325699,Zhejiang Province,China)

机构地区：[1]浙江省农业科学院植物保护与微生物研究所,杭州310021 [2]乐清市农业农村发展中心植物保护科,浙江乐清325699

出　　处：《植物保护学报》2021年第6期1303-1309,共7页Journal of Plant Protection

基　　金：浙江省择优委托项目(2020C02001)。

摘　　要：为有效防控有翅型小麦禾谷缢管蚜Rhopalosiphum padi,以浙江省小麦主要害虫禾谷缢管蚜为研究对象,比较分析其有翅型和无翅型转录组之间的差异,筛选差异表达基因,并对差异表达基因进行功能注释和代谢通路分析,采用实时荧光定量PCR(real-time fluorescence quantification PCR,RT-qPCR)技术对转录组测序结果进行验证。结果显示,经过StringTie软件组装,共获得39699条unigenes。注释到NCBI-NR、GO、KEGG、Pfam、Swiss-Prot和eggNOG六个数据库中unigenes数量分别为24120、17351、17137、17532、15494和23128条,分别占unigenes总数量的60.76%、43.71%、43.17%、44.16%、39.03%和58.26%。有翅型和无翅型禾谷缢管蚜之间共筛选到6747个基因差异表达显著,其中289个基因上调表达,其他6458个基因下调表达,大多数差异表达基因集中在糖代谢及氨基酸代谢等信号通路上,表明禾谷缢管蚜翅型分化可能与能量分配有关。UGT、MME、GST和ABCG14四个基因的RT-qPCR测定结果与转录组测定结果一致,表明转录组测序数据准确可信。In order to effectively control winged bird cherry-oat aphid Rhopalosiphum padi,the main wheat pest in Zhejiang Province,the transcriptomic differences between winged and wingless R.padi were compared and analyzed,and the differentially expressed genes were screened,their functions annotated,their metabolic pathways analyzed,and the transcriptome sequencing results verified with realtime fluorescence quantitative PCR(RT-qPCR).The results showed that a total of 39699 unigenes were obtained after StringTie software assembly.The number of unigenes annotated in NCBI-NR,GO,KEGG,Pfam,Swiss-Prot and eggNOG databases were 24120,17351,17137,17532,15494 and23128,respectively,accounting for 60.76%,43.71%,43.17%,44.16%,39.03% and 58.26% of the total number of unigenes,respectively.A total of 6747 genes were screened between winged and wingless R.padi,of which 289 genes were up-regulated and the other 6458 genes were down-regulated.Most of the differentially expressed genes were concentrated in the glucose metabolism and amino acid metabolism signal pathways,indicating that the differentiation of wing type in R.padi was probably related to energy distribution.The change trend in the expression of UGT,MME,GST and ABCG14 genes measured by RT-qPCR was consistent with that measured by transcriptome sequencing,suggesting that the transcriptome sequencing data were accurate and reliable.

关 键 词：麦蚜 翅型分化 转录组 基因表达 禾谷缢管蚜 

分 类 号：S435.122.2[农业科学—农业昆虫与害虫防治]