BMPR-IB、BMP15、ESR、GDF9与ADAMTS1基因多态性与杜泊羊繁殖性状的关联性分析  

Association Analysis of Gene Polymorphism of BMPR-IB,BMP15,ESR,GDF9,ADAMTS1 and Reproductive Traits of Dorper Sheep

作　　者：孙新颖 李美玉[1] 孙鹏 颜硕 朱新远 董焕声[1] 潘庆杰[1] SUN Xinying;LI Meiyu;SUN Peng;YAN Shuo;ZHU Xinyuan;DONG Huansheng;PAN Qingjie(College of Animal Science and Technology,Qingdao Agricultural University,Shandong Qingdao 266109,China;Shandong Vocational and Technical College of Animal Husbandry and Veterinary Medicine,Shandong Weifang 261061,China)

机构地区：[1]青岛农业大学动物科技学院,山东青岛266109 [2]山东畜牧兽医职业技术学院,山东潍坊261061

出　　处：《中国畜牧杂志》2022年第1期84-90,共7页Chinese Journal of Animal Science

基　　金：山东省动物种质资源保护与利用研究创新团队项目(6682216034);山东省现代农业产业技术体系羊产业创新团队项目(SDAIT-10-03);山东省农业重大应用技术创新项目(6682217004)。

摘　　要：为揭示绵羊繁殖力的影响机制,利用分子标记辅助选择来快速提高绵羊的繁殖性能,本实验采用直接测序法对杜泊羊与湖羊群体5个基因中的突变位点进行了比较分析。结合测序后序列通过DNAStar软件预测蛋白质二级结构;统计基因型频率、基因频率、纯合度、杂合度和多态信息含量并将基因多态性与产羔数进行关联分析。结果表明:杜泊羊ESR2基因中的RS428438934位点处发生T→C突变,导致氨基酸发生了改变,CC与CT、TT与CT基因型之间产羔数差异显著;RS418841713在杜泊羊与湖羊中都具有多态性,处于不平衡状态;RS398521635、RS402413016、G7、G8、RS417060437、RS604746425在杜泊羊与湖羊中均只有1种基因型,无突变类型,处于Hardy-Weinberg平衡状态。In order to reveal the influence mechanism of sheep's fecundity and use molecular marker-assisted selection to quickly improve sheep's reproductive performance,this experiment used direct sequencing method to compare and analyze the mutation sites in 5 genes of Dorper sheep and Hu sheep population.Combined with the sequence after sequencing,the protein secondary structure is predicted by DNAStar software;the genotype frequency,gene frequency,homozygosity,heterozygosity and polymorphism information content are counted,and the genetic polymorphism is correlated with litter size.The results showed that the T→C mutation in the RS428438934 locus in the ESR2 gene of Dorper sheep resulted in changes in amino acids.There was a significant difference in litter size between CC and CT,and TT and CT genotypes;RS418841713 Both Dorper sheep and Hu sheep have polymorphisms and are in an unbalanced state;RS398521635,RS402413016,G7,G8,RS417060437,RS604746425 have only one genotype in both Dorper sheep and Hu sheep,and there is no mutation type,and they are in Hardy-Weinberg balance state.

关 键 词：杜泊羊 湖羊 基因位点 多态性 

分 类 号：S826.2[农业科学—畜牧学]