模式动物(果蝇)体重高低杂种优势组合的转录组差异表达分析  

Heterosis of Body Weight in Line Crossing of Drosophila Melanogaster

作　　者：孙新宇 王珏 凌遥[1] 鲍海港[1] 吴常信[1] SUN Xinyu;WANG Jue;LING Yao;BAO Haigang;WU Changxin(College of Animal Science and Technology,China Agricultural University,Beijing 100193,China)

机构地区：[1]中国农业大学动物科学技术学院,北京100193

出　　处：《中国畜牧杂志》2022年第1期91-97,共7页Chinese Journal of Animal Science

摘　　要：本研究以5个果蝇突变品系(e、dp、vg、w、y)为研究材料进行5×5完全双列杂交,得到的二元杂交后代记为F1,共25种杂交组合,分别为ee、edp、evg、ew、ey等杂交组合;再根据纯系亲本和F1体重数据得出F1的体重性状杂种优势,筛选出3个体重高杂种优势组合(y♂vg♀、dp♂w♀、♂w♀e)和3个体重低杂种优势组合(vg♂dp♀、dp♂y♀、♂w♀dp);接着通过转录组测序分别筛选出2524个体重高杂种优势组合和体重低杂种优势组合的差异表达基因,最后利用GO和KEGG数据库对2524个差异表达基因进行基因功能富集和聚类分析以深入探究其运行通路和作用机制,结果显示糖代谢通路、脂代谢通路、谷胱甘肽和抗坏血酸代谢有关通路等被显著富集到,为进一步研究果蝇杂种优势的分子机理提供参考。果蝇体重高杂种优势的形成与糖代谢、氧化还原活动、抗氧化等相关基因功能和通路有着密切关系。In this study,five mutant lines of drosophila(e,dp,vg,w,y)were used for 5×5 complete diallel cross,and the binary hybrid offspring were denoted as F1,and they were ee,edp,evg,ew,ey and other cross combinations.With the weight of the corresponding pure line parents and the binary hybrid offspring F1,calculate the heterosis of body weight of F1 and filter out the combination of the high heterosis(y♂vg♀,dp♂w♀,♂w♀e)and the low hybrid combination(vg♂dp♀,dp♂y♀,♂w♀dp).2524 differential expression genes were selected by RNA-sequencing by the data of the high hybrid advantage combination and the low hybrid advantage combination,using GO and KEGG to analyze the function and pathway of the different expression genes to explore their mechanism,and thus provide further research background for the theoretical basis of the hybrid advantage and the result showed that glucose metabolic pathways,lipid metabolic pathways,glutathione and ascorbic acid metabolic pathways were significantly enriched.The formation of high heterosis was closely related to the gene function and pathways of sugar metabolism,oxidation reduction and oxidation resistance.

关 键 词：杂种优势 黑腹果蝇 体重 抗氧化 

分 类 号：S813[农业科学—畜牧学]