2020年昆明市儿童医院非伤寒沙门菌分子分型和β内酰胺类耐药基因特征分析  

Molecular typing of non-typhoid Salmonella and analysis ofβ-lactam resistance gene characteristics in Kunming Children’s Hospital in 2020

作　　者：王海平 许彬[1] 黄海林[1] 李珏 李小娟[1] 欧晓燕 蒋立 WANG Haiping;XU Bin;HUANG Hailin;LI Jue;LI Xiaojuan;OU Xiaoyan;JIANG Li(Department of Clinical Laboratory,Kunming Children’s Hospital,Kunming,Yunnan,China,650100)

机构地区：[1]昆明市儿童医院检验科,云南昆明650100

出　　处：《分子诊断与治疗杂志》2022年第1期32-35,共4页Journal of Molecular Diagnostics and Therapy

基　　金：云南省教育厅科学研究基金(2021J0287);昆明市卫生健康委员会卫生科研课题项目(20201101112);昆明市“春城计划”高层次人才引进工程青年人才专项。

摘　　要：目的分析2020年昆明市腹泻患儿中分离的非伤寒沙门菌株的分子分型及耐药基因特征,为儿童非伤寒沙门菌感染防治提供参考依据。方法收集2020年1至12月昆明市儿童医院实验室确诊的41例沙门菌感染病例的相关信息,并对分离到的菌株进行血清型分型及脉冲场凝胶电泳分型分析;在此基础上对菌株进行药敏试验及B内酰胺类耐药基因高通量荧光定量PCR检测。结果2020年昆明市儿童医院确诊的41例沙门菌感染患者主要集中在夏秋季,1岁以下儿童占到了确诊数的44%,男性患儿明显高于女性;在分离的41株沙门菌中,30株为鼠伤寒沙门菌,部分菌株分子分型相似性系数较高;分离的鼠伤寒沙门菌对部分青霉素类及氨基糖苷类抗生素耐药率达到了100%,β内酰胺类耐药基因blaTEM及blaCTX-M3/6、blaOXA1在分析的菌株中携带表达较高。结论鼠伤寒沙门菌是昆明市地区儿童非伤寒沙门菌感染的主要病原体,菌株间较高的同源性提示患者有相同暴露源的可能,携带耐药基因blaTEM及blaCTX-M3/6、blaOXA1是造成菌株多重耐药的主要原因之一。Objective To analyze the molecular typing and drug resistance gene characteristics of non-typhoid Salmonella strains isolated from children with diarrhea in Kunming in 2020,to provide reference for the prevention and control of non-typhoid Salmonella infection in children.Methods The information of 41 children with salmonella infection in Kunming Children’s Hospital was collected in 2020,and the isolates were serotyped and analyzed by pulsed field gel electrophoresis.B ased on the results,the drug sensitivity test and highthroughput fluorescence quantitative PCR detection ofβlacamine-related drug resistance genes were carried out on the strains.Results In 2020,the 41 cases of Salmonella infection confirmed by Kunming Children’s Hospital were mainly concentrated in summer and autumn,and children under 1 year old accounted for 44%of the confirmed cases,and male children were significantly higher than women.Among the 41 isolated Salmonella strains,30 were Salmonella typhimurium,and some strains had higher molecular typing similarity coefficients.The isolated Salmonella typhimurium was resistant to some penicillin and aminoglycoside antibiotics up to 100%,and the B-lactam resistance genes blaTEM,blaCTX-M3/6 and blaOXA1 were highly expressed in the analyzed strains.Conclusions Salmonella typhimurium is the main pathogen of non-typhoid Salmonella infection in children in Kunming.The high homology among the strains suggests that the patients may have the same exposure source,and the drug-resistant genes blaTEM,blaCTX-M3/6 and blaOXAl are one of the main reasons for the multiple drug resistance of the strains.

关 键 词：非伤寒沙门菌 分子分型 耐药基因 儿童 

分 类 号：R446.5[医药卫生—诊断学] R725.1[医药卫生—临床医学]