胎盘组织中IGFBP-1、VCAM-1、AQP3与妊娠期糖尿病的相关性  

Correlation between IGFBP-1,VCAM-1 and AQP3 in placental tissues and gestational diabetes mellitus

作　　者：王娜[1] 张小环 李燕[1] 邱雪洲 兰岚[1] WANG Na;ZHANG Xiaohuan;LI Yan;QIU Xuezhou;LAN Lan(Department of Obstetrics,Nanyang Central Hospital Affiliated to Zhengzhou University,Nanyang,Henan,China,473000)

机构地区：[1]郑州大学附属南阳市中心医院产科,河南南阳473000

出　　处：《分子诊断与治疗杂志》2022年第1期36-39,44,共5页Journal of Molecular Diagnostics and Therapy

基　　金：河南省科技发展计划项目(172102310172)。

摘　　要：目的探究妊娠期糖尿病(GDM)患者胎盘组织中胰岛素生长因子结合蛋白1(IGFBP-1)、血管细胞黏附分子-1(VCAM-1)、水通道蛋白3(AQP3)与胰岛素抵抗及妊娠结局的关系。方法选取郑州大学附属南阳市中心医院120例单胎妊娠的GDM患者(GDM组)及同期接受分娩的80例糖耐量正常者(健康对照组)为研究受试者。分娩后收集胎盘组织1 g,检测并比较两组受试者IGFBP-1、VCAM-1、AQP3基因表达差异及胰岛素抵抗指数(HOMA-IR)、胰岛β细胞功能指数(HOMA-β)水平,经Pearson相关分析评估GDM患者IGFBP-1、VCAM-1、AQP3基因表达与胰岛素指标的相关性。根据GDM组不良妊娠结局发生情况将其分为结局正常组及结局异常组两亚组,分别比较不同妊娠结局亚组间IGFBP-1、VCAM-1、AQP3基因表达差异,经Spearman相关分析评估GDM患者IGFBP-1、VCAM-1、AQP3基因表达与妊娠结局之间的相关性。结果GDM组胎盘组织中IGFBP-1、AQP3的mRNA相对表达量及HOMA-β水平显著低于健康对照组,VCAM-1的mRNA相对表达量、HOMA-IR水平显著高于健康对照组,差异有统计学意义(P<0.05);经Pearson相关分析,GDM组患者IGFBP-1、AQP3的mRNA相对表达量与HOMA-IR均具有负相关性(P<0.05),与HOMA-β呈正相关性(P<0.05),而VCAM-1与HOMA-IR具有正相关性(P<0.05),与HOM A-β呈负相关性(P<0.05);GDM组胎盘组织中IGFBP-1、AQP3的mRNA相对表达量显著低于健康对照组,VCAM-1的mRNA相对表达量显著高于健康对照组,差异有统计学意义(P<0.05);经Spearman相关分析,GDM组患者妊娠结局与IGFBP-1、AQP3的mRNA相对表达量具有负相关性(P<0.05),与VCAM-1 mRNA相对表达量具有正相关性(P<0.05)。结论胎盘组织中IGFBP-1、VCAM-1、AQP3对评估GDM患者胰岛素抵抗及妊娠结局有重要意义。Objective To explore the relationship between insulin-like growth factor binding protein-1(IGFBP-1),vascular cell adhesion molecule-1(VCAM-1)and aquaporin 3(AQP3)in placental tissues and insulin resistance and pregnancy outcomes of patients with gestational diabetes mellitus(GDM).Methods A total of 120 GDM patients with singleton pregnancies(GDM group)and 80 patients with normal glucose tolerance who received delivery during the same period(healthy control group)were selected as the research subj ects.1 g placental tissue was collected after delivery,and the gene expressions of IGFBP-1,VCAM-1 and AQP3 and levels of homeostasis model assessment of insulin resistance(HOMA-IR)and homeostasis model assessment ofβ-cell function(HOMA-β)were detected and compared between the two groups.The correlation between gene expressions of IGFBP-1,VCAM-1 and AQP3 and insulin indicators in GDM patients was evaluated by Pearson correlation analysis.According to the occurrence of adverse pregnancy outcomes in GDM group,they were divided into the normal outcome subgroup and abnormal outcome subgroup.The differences in gene expressions of IGFBP-1,VCAM-1 and AQP3 were compared between different pregnancy outcome subgroups,and the correlation between gene expressions of IGFBP-1,VCAM-1 and AQP3 and pregnancy outcomes in GDM patients was evaluated by Spearman correlation analysis.Results The relative mRNA expression of IGFBP-1 and AQP3 and the level of HOMA-βin placental tissues in the GDM group were significantly lower than those in the healthy control group,while the relative mRNA expression of VCAM-1 and the level of HOMA-IR were significantly higher than those in the healthy control group(P<0.05).The Pearson correlation analysis showed that the relative mRNA expressions of IGFBP-1 and AQP3 in the GDM group were negatively correlated with HOMA-IR(P<0.05),and positively correlated with HOMA-β(P<0.05).However,VCAM-1 had a positive correlation with HOMA-IR(P<0.05),and a negative correlation with HOMA-β(P<0.05).The relative mRNA expres

关 键 词：妊娠期糖尿病 胰岛素生长因子结合蛋白1 血管细胞黏附分子-1 水通道蛋白3 

分 类 号：R714.256[医药卫生—妇产科学]