两种品牌血培养瓶抗生素吸附能力对比研究  

Comparative study on antibiotic adsorption capacity of two brands of blood culture bottles

作　　者：王萌 周义正[2] 罗金柱[2] 李承彬[1] 龚权 WANG Meng;ZHOU Yizheng;LUO Jinzhu;LI Chengbin;GONG Quan(Department of Laboratory Medicine,Jingzhou Hospital Affiliated to Yangtze University,Jingzhou,Hubei,China,434020;Department of Laboratory Medicine,Jingzhou Central Hospital,Jingzhou,Hubei,China,434020;Yangtze University School of Medicine,Jingzhou,Hubei,China,433023)

机构地区：[1]长江大学附属荆州医院医学检验部,湖北荆州434020 [2]湖北省荆州市中心医院医学检验部,湖北荆州434020 [3]长江大学医学部,湖北荆州434023

出　　处：《分子诊断与治疗杂志》2022年第1期73-76,81,共5页Journal of Molecular Diagnostics and Therapy

基　　金：荆州市2020年度科技计划项目(2020CB2021-15);2019年湖北省中央引导地方科技发展专项(2019ZYYD066)。

摘　　要：目的比较美国BD Bactec树脂需氧瓶和溶血素厌氧瓶与法国梅里埃BacT/Alert树脂血培养瓶的抗生素吸附能力。方法选用临床常用抗生素:亚胺培南、万古霉素、氨苄西林、头孢曲松、甲硝唑、美罗培南、米卡芬净,按照一定浓度与标准菌株和无菌羊血混合,同时接种于2种品牌血培养瓶,记录不同血培养瓶五天内的阳性报警情况及检出时间,对比各血培养瓶的抗生素吸附能力。结果需氧瓶组:氨苄西林BMX-FA与BD-FA瓶检出率相同,均为100%,且BMX-FA较BD-FA瓶检出时间长(11.04±0.24 h vs 10.45±0.45 h),差异有统计学意义(t=2.59,P<0.05);含亚胺培南及头孢曲松组中,2种血培养瓶均未检出;含万古霉素组,BD-FA 比BMX-FA瓶的检出率低,分别为40%和100%,且BMX-FA 比BD-FA瓶的检出时间更短(15.70±0.76 h vs 21.50±0.71 h)差异有统计学意义(P<0.05);含米卡芬净组,BD-FA 比 BMX-FA瓶的检出率低,分别为60%和100%,且BMX-FA比BD-FA瓶检出时间更短(61.06±10.42 h vs 94.20±1.74h),差异有统计学意义(t=12.49,7.01,P<0.05)。厌氧瓶组:含亚胺培南、万古霉素、氨苄西林、甲硝唑、美罗培南的BD-FN瓶均未检出,BMX-FN瓶检出率均为100%;含头孢曲松组,2种血培养瓶均未检出。结论法国梅里埃BacT/Alert树脂血培养瓶的抗生素吸附能力总体上优于美国BD Bactec树脂需氧瓶和溶血素厌氧瓶,临床可根据抗生素使用情况选择合适的血培养瓶。Objective To compare the antibiotic adsorption capacity of American BD Bactec resin aerobic and Bactec-Lytic/10 anaerobic bottles with French bioMerieux BacT/Alert resin blood culture bottles.Methods The commonly used antibiotics of imipenem,vancomycin,ampicillin,ceftriaxone,metronidazole,meropenem,micafungin were applied in this study.According to a certain concentration,the standard strains and sterile sheep blood were mixed and inoculated into two brands of blood culture bottles at the same time.recorded the positive alarm conditions and detection time of the different blood culture bottles within five days,compared the antibiotic adsorption capacity of each blood culture bottle.Results Aerobic bottle group:Ampicillin BMX-FA and BD-FA bottles have the same detection rate of 100%,and BMX-FA has a longer detection time than BD-FA bottles(11.04±0.24 vs 10.45±0.45 h),and the differences were statistically significant(t=2.59,P<0.05).In the imipenem and ceftriaxone groups,the two blood culture bottles were not detected.In the vancomycin groups,the detection rate of BD-FA bottles was lower than Bthat of BMX-FA bottles,which were 40% and 100%,the time to detection of BMX-FA was shorter than BD-FA bottles(15.70±0.76 vs 21.50±0.71 h).In the micafungin groups,the detection rate of BD-FA bottles was lower than that of BMXFA bottles,which were 60% and 100%,and the detection time of BMX-FA was shorter than that of BD-FA bottles(61.06±10.42 vs 94.20±1.74 h),and the differences were statistically significant(t=12.49,7.01,P<0.05).naerobic bottles group:BD-FN bottles containing imipenem,vancomycin,ampicillin,metronidazole,meropenem were not detected,the detection rates of BMX-FN bottles were 100%.In the group con taining ceftriaxone,neither of the two blood culture bottles was detected.Conclusion The antibiotic adsorption capacity of B acT/Alert resin blood culture bottles was generally better than that of B actec resin aerobic and B actec-Lytic/10 anaerobic bottles,and the appropriate blood culture bottles can be selecte

关 键 词：树脂血培养瓶 抗生素吸附能力 检出时间 血流感染 

分 类 号：R446.5[医药卫生—诊断学]