联合cTnI、Mb和miR-133检测在AVM患儿诊断及预后中的作用  

Application of combined detection of cTnI,Mb and miR-133 in the diagnosis and prognosis of children with AVM

作　　者：张小建 张军毅[1] 冯嵩[1] 李丹[1] ZHANG Xiaojian;ZHANG Junyi;FENG Song;LI Dan(Department of Division 2 of Pediatric Internal Medicine,the First Affiliated Hospital of Zhengzhou University,Zhengzhou,Henan,China,450052)

机构地区：[1]郑州大学第一附属医院小儿内科2病区,河南郑州450052

出　　处：《分子诊断与治疗杂志》2022年第1期145-148,共4页Journal of Molecular Diagnostics and Therapy

基　　金：河南省医学教育研究项目(Wjlx2017438)。

摘　　要：目的探讨联合心肌肌钙蛋白T(cTnT)、肌红蛋白(Mb)、微小核糖核酸-133(miR-133)检测在急性病毒性心肌炎(AVM)患儿诊断及预后中的作用。方法选取2017年5月至2020年4月期间郑州大学第一附属医院收治的136例AVM患儿为研究组,另选取同期健康体检的108例健康儿童为对照组。比较两组cTnI、Mb、miR-133水平。根据AVM患儿诊断后1年内预后情况将研究组分为预后不良组和预后良好组。绘制ROC曲线图,分析cTnI、Mb、miR-133联合检测对AVM的诊断价值,探究影响AVM患儿预后的独立危险因素。结果研究组cTnI、Mb表达水平高于对照组,miR-133表达水平低于对照组,差异均有统计学意义(P<0.05)。cTnI、Mb、miR-133三者联合检测对AVM诊断价值的AUC最大,为0.915(0.844~0.986)(P<0.05)。136例患儿中预后不良21例,预后良好115例。Logistic回归模型分析得:使用肾上腺素、QT间期延长、cTnI>0.09μg/L、Mb>80 ng/mL、miR-133<3.1是影响AVM患儿预后的独立危险因素(P<0.05)。结论cTnI、Mb、miR-133在AVM患儿中呈异常表达,联合检测三者可作为AVM临床诊断及预后观察的有效手段。Objective To investigate the effects of combined detection of cardiac troponin T(cTnT),myoglobin(Mb),and microRNA-133(miR-133)on the diagnosis and prognosis of children with acute viral myocarditis(AVM).Methods A total of 136 children with AVM admitted to the First Affiliated Hospital of Zhengzhou University from May 2017 to April 2020 were selected as the study group,and 108 healthy children who underwent health examinations during the same period were selected as the control group.According to the prognosis of children with AVM within 1 year after diagnosis,they were divided into:the poor prognosis group(n=21)and the good prognosis group(n=115).The levels of cTnI,Mb and miR-133 in the two groups were compared.The ROC curve was drawn to analyze the diagnostic value of the combined detection of cTnI,Mb and miR-133 for AVM,and to explore the independent risk factors affecting the prognosis of children with AVM.Results The expression levels of cTnI and Mb in the study group were higher than those in the control group,and the expression level of miR-133 was lower than that in the control group,and the differences were statistically significant(P<0.05).The AUC of the combined detection of cTnI,Mb and miR-133 in the diagnosis of AVM was the largest,which was 0.915(0.844-0.986)(P<0.05).Among the 136 children,21 had poor prognosis and 115 had good prognosis.Logistic regression model analysis showed that epinephrine,QT interval prolongation,cTnI>0.09μg/L,Mb>80 ng/mL,and miR-133<3.1 were independent risk factors affecting the prognosis of children with AVM(P<0.05).Conclusion cTnl,Mb,and miR-133 are abnormally expressed in children with AVM.The combined detection of the three can be used as an effective means for clinical diagnosis and prognosis observation of AVM.

关 键 词：miR-133 MB AVM CTNI 

分 类 号：R725.4[医药卫生—儿科]