IGFBP-3基因核苷酸变异与绵羊屠宰性状相关性分析  

Correlation Analysis of IGFBP-3 Gene Nucleotide Variation and Ovine Slaughter Traits

作　　者：石丰运[1] 朱广琴[1] 廖云琼[1] 王兵[1] SHI Fengyun;ZHU Guangqin;LIAO Yunqiong;WANG Bing(School of Animal Engineering,Xuzhou Bioengineering Vocational and Technical College,Xuzhou 221151,China)

机构地区：[1]徐州生物工程职业技术学院动物工程学院,江苏徐州221151

出　　处：《华北农学报》2021年第S01期368-372,共5页Acta Agriculturae Boreali-Sinica

基　　金：徐州市科技计划项目(KC19119)。

摘　　要：胰岛素样生长因子结合蛋白与动物的生长发育有密切的联系。为了探讨IGFBP-3基因作为绵羊屠宰性状候选基因的可能性,采用单链构象多态性结合测序方法检测592只甘肃高山细毛羊IGFBP-3基因的变异特征,并分析核苷酸变异与屠宰性状的相关性。结果表明,绵羊IGFBP-3第4外显子区检测到1个单核苷酸变异位点(g.87 A>C),A和B 2个等位基因(等位基因频率:56.76%,43.24%),表现为AA、AB和BB 3种基因型(基因型频率:29.73%,54.05%,16.22%),并且所研究的群体在该位点上处于Hardy-Weinberg平衡状态(P>0.05)。相关性结果表明,甘肃高山细毛羊IGFBP-3基因g.87 A>C处的变异对宰前活质量、热胴体质量有极显著影响(P<0.01);对眼肌面积、GR值、嫩度有显著影响(P<0.05)。表明等位基因B的绵羊群体具有更高的屠宰率,可以作为甘肃高山细毛羊屠宰性状选育的分子标记用于生产实践中。Insulin-like Growth Factor Binding Proteins(IGFBPs)are closely related to the growth and development of animals.In order to explore the possibility of IGFBP-3 gene as a candidate gene for sheep slaughter traits,this study used Polymerase Chain Reaction-Single Strand Conformation Polymorphism(PCR-SSCP)combined with sequencing to detect variation characteristics of IGFBP-3 gene and to explore the correlation between nucleotide variation and slaughter traits in 592 Gansu alpine merino sheep.The results showed that one single nucleotide variant site(g.87 A>C)was detected in the fourth exon region of sheep IGFBP-3,and two alleles A and B(alle frequency:56.76%and 43.24%),showing three genotypes of AA,AB and BB(genotype frequency:29.73%,54.05%,16.22%),and the population studied was in the Hardy-Weinberg equilibrium state at this locus(P>0.05).The correlation results showed that the mutation at g.87 A>C of Gansu alpine merino sheep IGFBP-3 gene had a very significant effect on live weight and hot carcass weight before slaughter(P<0.01);it had a significant effect on eye muscle area,GR value and tenderness.Degree had a significant impact(P<0.05).It showed that the sheep population of allele B had a higher slaughter rate,and it could be used as a molecular marker for the selection of slaughter traits of Gansu alpine merino sheep in production practice.

关 键 词：甘肃高山细毛羊 IGFBP-3基因 变异 屠宰性状 

分 类 号：Q78[生物学—分子生物学] S826[农业科学—畜牧学]