SPE-HPLC测定重症感染患者血浆中利奈唑胺浓度  

Determination of Linezolid in Plasma of Severely Infected Patients by SPE-HPLC

作　　者：刘义钊[1] 李娜梅 周覃 沈毅[1] 彭学清[1] LIU Yi-zhao;LI Na-mei;ZHOU Qin;SHEN Yi;PENG Xue-qing(The First People's Hospital of Changde City,Changde 415100,China)

机构地区：[1]常德市第一人民医院,湖南常德415000

出　　处：《海峡药学》2021年第12期84-86,共3页Strait Pharmaceutical Journal

基　　金：常德市科技计划(2019S180)。

摘　　要：目的建立SPE-HPLC法检测重症患者血浆中利奈唑胺浓度,并应用于临床。方法以氯霉素为内标,色谱柱为Welch materials XB-C_(18)(150 mm×4.6 mm,5μm),流动相为甲醇-乙腈-水(15∶20∶65),流速1.0 mL·min^(-1),柱温30℃,检测波长254 nm。结果利奈唑胺在质量浓度0.25~40μg·mL^(-1)内线性关系良好(r=0.9999),最低定量限为0.25μg·mL^(-1);高、中、低浓度的样品日间、日内RSD均<10%,相对回收率分别为115.61%、98.88%、99.88%,绝对回收率>90%。结论本方法简便,灵敏、快速,适用于重症感染患者血浆中利奈唑胺的检测,并为其个体化给药方案调整提供依据。OBJECTIVE To establish a SPE-HPLC method for determining the concentration of linezolid in severely infected patients plasma.METHODS With chloromycetin as internal standard,the sample was separated on a Welch materials XB-C_(18) column.The mobile phase was consisted of Methanol-acetonitrile-water(15:20:65)with a flow rate of 1.0 mL·min^(-1) at 30℃.The detection wavelength was set at 254 nm.RESULTS Excellent linear relationship was obtained from 0.25 to 40 μg·mL^(-1)(r=0.9999).The lowest limit of quantification was 0.25 μg·mL^(-1).The intra-day and inter-day precisions were both less than 10%.For the three check samples,the relative re-coveries were 115.61%,98.88%,99.88% respectively and absolute recoveries were more than 90%.CONCLUSION The established HPLC method is sensitive,fast and accurate.This method is suitable for determination of linezolid in severely infected patients plasma and it can be used for individualized drug delivery program study.

关 键 词：利奈唑胺 重症感染患者 固相萃取-高效液相色谱法 

分 类 号：R969.1[医药卫生—药理学]