ω-黑麦碱基因表达缺失的1BL/1RS易位系种质创新  

Germplasm Innovation on 1BL/1RS Translocations withω-Secalin Absence

作　　者：张士昌[1] 李亚青[1] 张楠[1] 彭义峰[1] 李孟军[1] ZHANG Shichang;LI Yaqing;ZHANG Nan;PENG Yifeng;LI Mengjun(Shijiazhuang Academy of Agriculture and Forestry Sciences/Wheat Engineering Research Center of Hebei Province,Shijiazhuang,Hebei 050041,China)

机构地区：[1]石家庄市农林科学研究院/河北省小麦工程技术研究中心,河北石家庄050041

出　　处：《麦类作物学报》2022年第1期11-16,共6页Journal of Triticeae Crops

基　　金：石家庄市科学技术研究与发展计划项目(191490332A);河北省重点研发计划项目(20326346D)。

摘　　要：1BL/1RS易位系是小麦育种的重要亲本,但1RS Sec-1位点表达的ω-黑麦碱是导致小麦加工品质不佳的重要因素。为消除ω-黑麦碱的不良影响,进一步挖掘1BL/1RS易位系的育种潜力,本研究利用低能N+离子束处理1BL/1RS易位系小麦干种子,利用醇溶蛋白酸性聚丙烯酰胺凝胶电泳(A-PAGE)筛选ω-黑麦碱表达缺失突变株系,1RS染色体特异引物鉴定携带1RS染色体的突变株系,结果共创制出9个ω-黑麦碱基因Sec-1位点表达缺失的新的1BL/1RS易位系种质。农艺性状和品质性状分析结果表明,这些1BL/1RS易位系新种质的籽粒蛋白含量、面筋指数、稳定时间等加工品质性状显著提高,株高、穗数、千粒重等农艺性状无显著变化。ω-黑麦碱表达缺失突变体的育成为培育优质抗逆小麦品种提供了新的方法。The 1 BL/1 RS translocation lines are the important parents in Chinese wheat breeding.However,ω-secalins encoded by Sec-1 locus on IRS chromosome have a negative impact on processing quality of 1 BL/1 RS translocation wheat lines.In order to remove the negative effects ofω-secalins and excavate breeding potential of 1 BL/1 RS translocation lines,the dry seeds of 1 BL/1 RS translocation lines were firstly treated with nitrogen ion beam.Acid polyacrylamide gel electrophoresis was used for screening 1 BL/1 RS translocation lines withω-secalin absence.Finally the mutation lines carrying IRS chromosome were identified using IRS-specific markers,and nine new 1 BL/1 RS translocation germplasms were developed.The processing quality of these new germplasms incuding grain protein content,gluten index and dough stability time was improved,while agronomic characters,including plant height,grain numbers and 1000-grain weight,had no significant difference.The development of 1 BL/1 RS translocation lines withω-secalin absence provided a new way for breeding new high-quality wheat varieties with high stress-resistance.

关 键 词：1BL/1RS易位系 ω-黑麦碱 加工品质 农艺性状 

分 类 号：S512.1[农业科学—作物学] S330