碳纤维-纳米金复合材料修饰电极检测ATP的研究  

Application of Carbon Nanofiber-Gold Nanocomposite Modified Electrode for the ATP Detection

作　　者：张思月 邵波 谢慧[1] 胡亚晨 张冰雪 张晓萍 孙伟[1] ZHANG Siyue;SHAO Bo;XIE Hui;HU Yachen;ZHANG Bingxue;ZHANG Xiaoping;SUN Wei(Key Laboratory of Laser Technology and Optoelectronic Functional Materials of Hainan Province,Key Laboratory of Functional Materials and Photoelectrochemistry of Haikou,College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou 571158,China)

机构地区：[1]海南师范大学化学与化工学院,海南省激光技术与光电功能材料重点实验室,海口市功能材料与光电化学重点实验室,海南海口571158

出　　处：《海南师范大学学报（自然科学版）》2021年第4期371-376,共6页Journal of Hainan Normal University（Natural Science）

基　　金：海南省基础与应用基础研究计划(自然科学领域)高层次人才项目(220RC594,2019RC188);国家自然科学基金项目(21964007)。

摘　　要：采用静电纺丝法和高温碳化法制备了碳纤维材料,进一步用水热合成法制备碳纤维-纳米金复合材料。将优化浓度的碳纤维-纳米金复合材料作为修饰剂固定于碳离子液体电极(CILE)的表面制得了碳纤维-纳米金修饰电极(CNF-Au/CILE),采用扫描电子显微镜考察了复合材料的表面形貌。最优实验条件下将该修饰电极用于ATP的检测,结果发现ATP浓度为0.06~0.80 mmol/L和12.0~150.0 mmol/L时,氧化峰电流值与浓度呈现出良好的线性关系,检测限为0.023 mmol/L。常见干扰物质对ATP的测定影响较小,说明该方法具有良好的选择性,并成功应用于三磷酸腺苷二钠注射液中ATP含量的测定。Carbon nanofibers were prepared by electrospinning method and high temperature carbonization method,and carbon nanofiber-gold nanocomposite were prepared by hydrothermal method.The optimized concentration of carbon nanofiber-gold nanocomposite was fixed on the surface of carbon ionic liquid electrode(CILE),obtaining a CNF-Au/CILE modified electrode that was then observed by scanning electron microscopy.The modified electrode was used for the detection of ATP under the optimal experimental conditions.It was found that when the ATP concentration was 0.06~0.80 mmol/L and12.0~150.0 mmol/L,there was a good linear relationship between the oxidation peak current value and the concentration,and the detection limit for ATP was 0.023 mmol/L.Common interfering substances had little effects on the determination of ATP,indicating that this method had good selectivity,and it was successfully applied to the determination of ATP content in adenosine disodium triphosphate injection.

关 键 词：三磷酸腺苷 碳纤维 纳米金粒子 离子液体修饰碳糊电极 

分 类 号：O646.5[理学—物理化学]