西安市大田土壤中抗药性烟曲霉发生率调查  

Investigation on Incidence of Resistant Aspergillus fumigatus in Field Soil in Xi 'an

作　　者：崔宁 王培 许宁侠[1] CUI Ning;WANG Pei;XU Ningxia(Xi'an International University,Xi'an,Shaanxi 710077,China)

机构地区：[1]西安外事学院,陕西西安710077

出　　处：《陕西农业科学》2021年第12期85-88,共4页Shaanxi Journal of Agricultural Sciences

基　　金：陕西省教育厅科学研究计划项目(20JK0863);陕西省科技厅计划项目(2020JQ-916);西安外事学院高层次人才启动基金项目(XAIU2019013)。

摘　　要：为了探究农用三唑类化合物的使用与烟曲霉抗药性之间的潜在联系,研究采集了西安市区周边大田土壤样品36份,其中15份土壤样品中共分离出34株烟曲霉菌株,烟曲霉菌株的分离率为41.7%。34株烟曲霉中有2株(R-5和R-17)表现出三唑类药物的抗药性,抗性菌株占烟曲霉菌株总数的5.9%。经基因测序结果表明菌株R-5其cyp51A基因无点位点突变,R-17菌株cyp51A基因存在TR34/L98H/S297T/F495I型突变。In order to explore the relationship between the agricultural triazole compounds and the resistance of Aspergillus fumigatus,36 soil samples from fields around the urban area of Xi'an were collected in this study.A total of 34 A.fumigatus strains were isolated from 15 soil samples,and the isolation rate of A.fumigatus strains was 41.7%.Two of 34 strains(R-5 and R-17)of A.fumigatus showed resistance to triazole,accounting for 5.9%of the total isolated strains.cyp51A gene had no mutation in strain R-5,while there were TR34/L98H/S297T/F495I mutation(one tandem repeats and three point mutations)in strain R-17.

关 键 词：烟曲霉 交互抗药性 农药 

分 类 号：S482[农业科学—农药学]