单肺通气诱发兔肺损伤时花生四烯酸代谢通路变化及丹参多酚酸盐的干预研究  

Changes of arachidonic acid metabolic pathway and intervention effect of salvianolic acid salt in OLV induced lung injury in rabbits

作　　者：陈华[1] 张柏盛[1] 龙小平 Chen Hua;Zhang Bosheng;Long Xiaoping(Dept of Traditional Chinese Medicine,The First Affiliated Hospital of Nanhua University,Hengyang 421001;Pulmonary and Critical Care Medicine,The First Affiliated Hospital of Nanhua University,Hengyang 421001)

机构地区：[1]南华大学附属第一医院中医科,衡阳421001 [2]南华大学附属第一医院呼吸与危重医学科,衡阳421001

出　　处：《安徽医科大学学报》2022年第1期21-25,共5页Acta Universitatis Medicinalis Anhui

基　　金：湖南省自然科学基金青年基金项目(编号:2018JJ3470)。

摘　　要：目的探讨丹参多酚酸盐对单肺通气(OLV)致兔肺损伤后的保护作用及机制。方法24只白兔随机分为3组:对照、模型和治疗组,每组8只。测定肺湿/干值(W/D值),通过HE染色及电镜观察肺组织变化;采用ELISA法检测兔肺组织中花生四烯酸(AA)、白三烯B4(LTB4)、血栓素A2(TXA2)和前列环素(PGI2)含量;采用RT-qPCR法检测肺组织环氧化酶-2(COX-2)和5-脂氧化酶(5-LOX)mRNA表达水平;采用Western blot法检测肺组织中COX-2、5-LOX、Clara细胞分泌蛋白(CCSP)和胞质型磷脂酶A2(C-PLA2)蛋白表达。结果模型组肺W/D比值较对照组升高,而治疗后下降(P<0.05);HE染色显示,模型组毛细血管扩张充血,肺泡腔内有红色渗出物,肺泡壁增厚,炎性细胞浸润,而丹参多酚酸盐治疗后有所减轻;透射电镜下观察模型组线粒体肿胀,嵴断裂甚至消失,治疗组AT-I超微结构损伤较模型组明显改善;模型组和治疗组肺组织AA、LTB4、TXA2和PGI2含量均高于对照组,经治疗后AA、LTB4、TXA2和PGI2含量下降(P<0.05);RT-qPCR结果显示,模型组COX-2、5-LOX mRNA表达均高于对照组(P<0.05),而治疗后表达下降(P<0.05);Western blot结果显示,治疗组COX-2、5-LOX和C-PLA2蛋白表达均低于模型组,而CCSP蛋白表达上升(P<0.05)。结论丹参多酚酸盐具有保护OLV所致肺损伤的作用,其机制可能与下调COX-2、5-LOX和C-PLA2表达,上调CCSP表达有关。Objective To explore the protective effect and mechanism of salvianolic acid salt on acute lung injury induced by one lung ventilation(OLV)in rabbits.Methods 24 rabbits were randomly divided into three groups:control group,model group and treatment group,with 8 rabbits in each group.The W/D value of lung was measured,and the morphological changes of lung tissue were observed by HE staining and electron microscopy;The contents of arachidonic acid(AA),leukotriene B4(LTB4),thromboxaneA2(TXA2)and prostacyclin 2(PGI2)in rabbit lung tissue were detected by ELISA;The expressions of cyclooxygenase-2(COX-2),5-lipoxygenase(5-LOX)in lung tissue were detected by RT-qPCR;The expressions of COX-2,5-LOX,clara cell secretory protein(CCSP)and cytoplasmic phospholipase A2(C-PLA2)in lung tissue were detected by Western blot.Results The W/D ratio of model group was higher than that of control group,but decreased after treatment(P<0.05);HE staining showed that in the model group,the capillaries were dilated and congested,there were red exudates in the alveoli,the walls of alveoli were thickened,and inflammatory cells were infiltrated,but the treatment of salvianolic acid salt was relieved;The mitochondria swelling,crista breaking and even disappearing were observed by transmission electron microscope in the model group,and alveolar epithelial cellⅠ(AT-Ⅰ)ultrastructural damage in the treatment group significantly improved compared with that in the model group;The AA,LTB4,TXA2 and PGI2 contents in the treatment group were lower than those in the model group(P<0.05).RT-qPCR showed that the expression of COX-2 and 5-LOX in model group was higher than that in control group(P<0.05),while the expression of COX-2 and 5-LOX decreased after Salvianolic acid salt treatment(P<0.05);Western blot showed that the expression of COX-2,5-LOX and C-PLA2 in treatment group was lower than that in model group,while the expression of CCSP increased(P<0.05).Conclusion Salvianolic acid salt has protective effect on lung injury induced by one-lung ventila

关 键 词：丹参多酚酸盐 单肺通气 环氧化酶-2 5-脂氧化酶 CLARA细胞分泌蛋白 胞质型磷脂酶A2 

分 类 号：R392[医药卫生—免疫学]