杨凌糖丝菌Hhs.015拮抗苹果树腐烂病菌转录组分析及抗菌机制探究  

Transcriptome Analysis and Antibacterial Mechanism of Saccharothrix yanglingensis Hhs. 015Antagonistic Valsa mali

作　　者：王建勋 康凤 孙玲 颜霞[1,2] 黄丽丽 WANG Jianxun;KANG Feng;SUN Ling;YAN Xia;HUANG Lili(College of Life Science,Northwest A&F University,Yangling Shanxi 712l00,China;State Key Laboratory of Crop Stress Biology for Arid Areas,Northwest A&F University,Yangling Shaanxi0712100,China;College of Plant Protection,Northwest A&F University,Yangling Shaanxi 712100,China)

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]国家旱区作物逆境生物学国家重点实验室,陕西杨凌712100 [3]西北农林科技大学植物保护学院,陕西杨凌712100

出　　处：《西北农业学报》2022年第1期105-116,共12页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家重点研发计划(2017YFD0200602-2);国家自然科学基金(32072477)。

摘　　要：杨凌糖丝菌Hhs.015是一株稀有放线菌,能够广谐高效地拮抗病原真菌。为了研究其抗菌机理,对Hhs.015拮抗苹果树腐烂病菌Valsa mali的过程进行转录组分析。鉴定到533个差异表达基因,其中310个基因上调表达,223个基因下调表达。在此过程中,杨凌糖丝菌Hhs.015的信号传递、能量供应、细胞壁合成等功能受到Valsa mali的抑制。通过碳水化合物,氨基酸等物质的代谢增强补充能量的供应,AEC和MFS家族蛋白对于次级代谢物的运输和Valsa mali毒性物质的排出起到重要作用,糖苷水解酶的大量上调表达破坏了Valsa mali细胞壁合成,抑制了Valsa mali的生长。还预测到5个未知的次级代谢物在抗菌过程中起到重要作用,这些物质可以被运用于生态友好型的农业发展中,加快可持续发展的进程。Saccharothrix yanglingensis strain Hhs.015 is a rare actinomycete that can antagonizepathogenic fungi with broad spectrum and high efficiency.In order to study its antifungal mechanism.transcriptome analysis was performed on the process of Hhs.015 antagonizing apple Valsa cankerpathogen Valsa mali.533 differentially expressed genes were identified,of which 310 genes were up-regulated and 223 were down-regulated.In this antagonistic process,the signal transmission,energysupply,cell wall synthesis and other functions of Saccharothrix yanglingensis Hhs.015 were inhibi-ted by Valsa mali,metabolizing carbohydrates,amino acids,and other substances as supplementaryenergy.ABC and MFS family proteins play important roles in the transportation of secondary metabo-lites and the discharge of Valsa mali toxic substances.The destruction of Valsa mali cell wall andgrowth inhibition were led by the massive up-regulation of glycoside hydrolase Saccharothrix yan-glingensis Hhs.015.The prediction of five unknown secondary metabolites plays an important role inthe antifungal process.These 5 substances can be utilized in the development of eco-friendly agricul-ture to accelerate the process of sustainable development.

关 键 词：杨凌糖丝菌 苹果树腐烂病菌 转录组分析 抗菌机理 次级代谢物 

分 类 号：Q939[生物学—微生物学]