外源Ca^(2+)缓解玉米幼苗盐胁迫伤害的研究  

Study on Alleviating Salt Stress Injury of Maize Seedlings by Exogenous Ca^(2+)

作　　者：邸桂俐[1] 徐艳梅 高超[1] 王建丽[1] 潘多锋[1] 康庆华[3] 姜卫东[3] 边亚娟[4] DI Gui-li;XU Yan-mei;GAO Chao;WANG Jian-li;PAN Duo-feng;KANG Qing-hua;JIANG Wei-dong;BIAN Ya-juan(Pratacultural Science Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;Guizhou Normal University,Guiyang 163712,China;Institute of Industrial Crops,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;Heilongjiang Vocational College of Biology Science and Technology,Harbin 150025,China)

机构地区：[1]黑龙江省农业科学院草业研究所,黑龙江哈尔滨150086 [2]贵州师范学院,贵州贵阳550000 [3]黑龙江省农业科学院经济作物研究所,黑龙江哈尔滨150086 [4]黑龙江生物科技职业学院,黑龙江哈尔滨150025

出　　处：《黑龙江农业科学》2022年第2期14-17,共4页Heilongjiang Agricultural Sciences

基　　金：黑龙江省农业科学院“农业科技创新跨越工程”专项(HNK2019CX08);黑龙江省科研院所科研业务费(CZYF2020C007)。

摘　　要：为改善大庆盐碱地土壤对农作物生长的抑制,促进耐盐玉米品种的选育,以龙育4号玉米为材料,对玉米幼苗进行NaCl盐胁迫处理,同时添加1,3和5 mmol·L^(-1)的Ca^(2+)缓解盐胁迫,测定玉米幼苗的丙二醛(MDA)、叶绿素含量、过氧化物酶(POD)、可溶性糖含量、可溶性蛋白含量和脯氨酸含量。结果表明:植物受到盐胁迫后与对照相比MDA含量显著增加,叶绿素含量显著降低,POD活性显著增强,渗透调节物质脯氨酸含量和可溶性蛋白增加不显著,但可溶性糖含量显著增加(P<0.05)。加入3 mmol·L^(-1)Ca^(2+)后,与盐胁迫处理相比,MDA、脯氨酸、可溶性蛋白含量及POD活性均降到最低,且与盐胁迫处理差异显著(P<0.05)。综上所述,外源Ca^(2+)对玉米幼苗盐胁迫伤害具有一定的缓解作用,3 mmol·L^(-1)Ca^(2+)处理对玉米幼苗盐胁迫缓解效果最佳。In order to improve the inhibition of saline alkali soil on crop growth in Daqing and promote the breeding of salt tolerant maize varieties,with maize Longyu 4 as the material,maize seedlings were treated with NaCl stress and alleviated by adding(1,3 and 5 mmol·L^(-1))concentrations of Ca^(2+),and the contents of malondialdehyde(MDA),chlorophyll,peroxidase(POD),soluble sugar,soluble protein and proline were measured.The results showed that the plant under salt stress compared with the control indicated the MDA content increased,chlorophyll content decreased,POD activity increased and soluble sugar content increased,significant differences(P<0.05).Proline and the content of soluble protein increased,but the differences were not significant.After adding 3 mmol·L^(-1)Ca^(2+),the contents of MDA,proline,soluble protein and POD activity decreased to the lowest level compared with salt stress group,and the differences were significant(P<0.05).In conclusion,exogenous Ca^(2+) has a certain alleviating effect on maize seedlings under salt stress,and 3 mmol·L^(-1)Ca^(2+)treatment has the best alleviating effect on maize seedlings under salt stress.

关 键 词：外源钙 缓解效应 玉米 盐胁迫 

分 类 号：S51[农业科学—作物学]