一株抗大豆胞囊线虫芽孢杆菌的分离鉴定  

Separation and Identification of Bacillus sp.Antagonistic to Soybean Cyst Nematode

作　　者：符堯 李静 张子安 周园园 FU Yao;LI Jing;ZHANG Zi-an;ZHOU Yuan-yuan(Agricultural College,Heilongjiang Bayi Agricultural University,Daqing 163316,China;National Coarse Cereals Engineering Research Center,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学农学院,黑龙江大庆163316 [2]国家杂粮工程技术研究中心,黑龙江大庆163319

出　　处：《黑龙江农业科学》2022年第2期36-40,共5页Heilongjiang Agricultural Sciences

基　　金：黑龙江省大学生创新创业训练计划(202010223105);大庆市指导性科技计划(zd-2021-72)。

摘　　要：为了筛选用作防治大豆胞囊线虫的微生态制剂的益生菌,从大豆根围土中分离芽孢杆菌Bal-7,并测定其发酵液对大豆胞囊线虫2龄幼虫活性的影响。结合形态学、生理生化特征及16S rRNA基因序列比对分析对菌株进行鉴定。结果表明:分离筛选的芽孢杆菌Ba1-7发酵液对大豆胞囊线虫2龄幼虫校正死亡率为83.32%,经分子生物学鉴定菌株Ba1-7为阿氏芽孢杆菌(Bacillus aryabhattai)。菌株Ba1-7可用作防治大豆胞囊线虫的生防菌剂。In order to screen biocontrol strains against soybean cyst nematode,Bacillus Ba1-7 was isolated from soybean rhizosphere soil,identified by morphological,physiological and biochemical characteristics and 16S rRNA gene sequence alignment analysis.The inhibition rate of the fermentation of the strain Ba1-7 on soybean cyst nematode were determined.The results showed that the isolated and screened Ba1-7 was Bacillus aryabhattai.The correted mortality rate of the Ba1-7 fermentation on the hatching of J2 was 83.32%.Strain Ba1-7 can be used as a biocontrol agent against soybean cyst nematode.

关 键 词：大豆胞囊线虫 芽孢杆菌 分离 鉴定 

分 类 号：S43[农业科学—农业昆虫与害虫防治]