某育肥羊场呼吸道疾病的诊断与病原学鉴定  

Diagnosis and pathogen identification of the respiratory disease in a fattening goat farm

作　　者：恽佳蕾 何苗峰 王少辉[4] 毛立[1] 杨蕾蕾[1] 张纹纹[1] 孙敏 刘茂军[1,2,3] 李文良 YUN Jialei;HE Miaofeng;WANG Shaohui;MAO Li;YANG Leilei;ZHANG Wenwen;SUN Min;LIU Maojun;LI Wenliang(Jiangsu Key Laboratory of Food Quality and Safety-National Key Laboratory Base Co-built by Jiangsu Province and Ministry of Agriculture/Key Laboratory of Veterinary Biological Products Engineering Technology,Ministry of Agriculture and Rural Affairs/Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,China;College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,China)

机构地区：[1]江苏省农业科学院兽医研究所/农业部兽用生物制品工程技术重点实验室/江苏省食品质量安全重点实验室-省部共建国家重点实验室培育基地,江苏南京210014 [2]江苏大学食品与生物工程学院,江苏镇江212013 [3]南京农业大学动物医学院,江苏南京210095 [4]中国农业科学院上海兽医研究所,上海200241

出　　处：《畜牧与兽医》2022年第1期94-99,共6页Animal Husbandry & Veterinary Medicine

基　　金：江苏省农业科技自主创新资金(CX(18)2003);“十三五”国家重点研发计划(2016YFD0500900)。

摘　　要：为明确江苏某羊场外购育肥羊突发呼吸道疾病的病因,本研究结合临床症状、剖检病变观察、病原学检测与分离鉴定等方法对病因进行分析。结果表明:该病是由肠道外致病性大肠杆菌、山羊副流感病毒3型及绵羊肺炎支原体混合感染所致。分离所得的2株大肠杆菌对多黏菌素B、强力霉素敏感,对其他12种常见抗生素均不敏感,检测到其携带有iss、fyuA、iroN、cvaC、fimH、iutA、kpsMTⅡ这7种毒力基因,分别属于系统进化群B1群与B2群。通过MDBK细胞成功分离获得1株山羊副流感病毒3型毒株,其M基因片段与流行毒株同源性最高达99.7%。该结果将有助于科学认识和防控山羊呼吸道疾病。In order to clarify the etiology of the respiratory disease in a fattening goat farm in Jiangsu Province,observation of clinical symptoms and pathogenic lesions,pathogens detection,isolation and identification were performed on the affected goats.The results showed that the disease was caused by co-infection of extra-intestinal pathogenic Escherichia coli(ExPEC),caprine parainfluenza virus type 3(CPIV3)and Mycoplasma ovinepneumoniae(Mo).The two isolated ExPEC strains were sensitive to polymyxin B and doxycycline,but not to the other twelve common antibiotics.They were detected to carry seven virulence genes,including iss,fyuA,iroN,cvaC,fimH,iutA and kpsMTⅡ,belonging to phylogenetic groups B1 and B2,respectively.A CPIV3 strain was successfully isolated from the MDBK cells,and its M gene fragment was 99%homologous to the prevalent strains.These results would be helpful for scientific understanding and prevention ofrespiratory diseases in goats in the future.

关 键 词：肠道外致病性大肠杆菌 山羊副流感病毒3型 绵羊肺炎支原体 混合感染 分离鉴定 

分 类 号：S858.26[农业科学—临床兽医学]