不同基原肉苁蓉的苯乙醇苷、环烯醚萜类特征图谱研究  

Study on the characteristic chromatograms of phenyle-thanoid glycosides and iridoid glycosides in Herba Cistanche of different origins

作　　者：黄雄梅 黄艳 农新维 韦敏灵 韦红言 HUANG Xiongmei;HUANG Yan;NONG Xinwei;WEI Minling;WEI Hongyan(PuraPharm(Nanning)Pharmaceuticals Limitid Company,Nanning 530007,China)

机构地区：[1]培力(南宁)药业有限公司,南宁530007

出　　处：《西北药学杂志》2021年第6期881-884,共4页Northwest Pharmaceutical Journal

基　　金：广西南宁市科学研究与技术开发计划项目(编号:20201048)。

摘　　要：目的建立肉苁蓉中苯乙醇苷、环烯醚萜类成分高效液相色谱(high performance liquid chromatography,HPLC)法特征图谱的质量评价方法,并比较不同基原肉苁蓉的HPLC特征图谱。方法采用Agilent ZORBAX Eclipse Plus C_(18)色谱柱(4.6 mm×250 mm,5μm);乙腈-1 mL·L^(-1)磷酸水溶液梯度洗脱;流速为1.0 mL·min^(-1);检测波长为240 nm;柱温为30℃。结果管花肉苁蓉呈现9个特征峰、肉苁蓉呈现10个特征峰、盐生肉苁蓉呈现7个特征峰、沙苁蓉呈现4个特征峰。峰6为松果菊苷、峰7为毛蕊花糖苷、峰8为管花苷A、峰9为异毛蕊花糖苷、峰10为肉苁蓉苷A。4种不同基原肉苁蓉的特征图谱均有显著差异。结论建立的特征图谱方法重复性好,可用于区分肉苁蓉、管花肉苁蓉、沙苁蓉及盐生肉苁蓉4种不同基原药材。Objective To establish a quality evaluation method for Herba Cistanche based on HPLC characteristic chromatograms of phenylethanoid glycosides and iridoid glycosides,and to compare the HPLC characteristic chromatograms of different origins of Herba Cistanche.Methods The chromatographic conditions were as follows:column,Agilent ZORBAX Eclipse Plus C_(18) column(4.6 mm×250 mm,5μm);gradient elution,acetonitrile-1 mL·L^(-1)phosphoric acid as mobile phase;flow rate was 1.0 mL·min^(-1);detection wavelength was 240 nm;column temperature was 30℃.Results Cistanche tubulosa(Schenk)Wight showed 9 characteristic peaks,Cistanche deserticola Y.C.Ma showed 10 characteristic peaks,Cistanche salsa(C.A.Mey.)G.Beck showed 7 characteristic peaks,and Cistanche sinensis G.Beck showed 4 characteristic peaks.The peaks 6-10 were echinacoside,verbascoside,tubuloside A,isoacteoside and cistanoside A,respectively.The characteristic chromatograms of 4 different origins of Herba Cistanche were significantly different.Conclusion This characteristic chromatogram method has good reproducibility and can be used to distinguish 4 different origins of Herba Cistanche,C.deserticola Y.C.Ma,C.tubulosa(Schenk)Wight,C.sinensis G.Beck and C.salsa(C.A.Mey.)G.Beck.

关 键 词：肉苁蓉 管花肉苁蓉 沙苁蓉 盐生肉苁蓉 特征图谱 高效液相色谱法 

分 类 号：R282[医药卫生—中药学]