Ultra-thin temperature controllable microwell array chip for continuous real-time high-resolution imaging of living single cells  

作　　者：Yuanyuan Wu Lei Zhao Yaran Chang Liang Zhao Guangsheng Guo Xiayan Wang 

机构地区：[1]Center of Excellence for Environmental Safety and Biological Effects,Beijing Key Laboratory for Green Catalysis and Separation,Department of Chemistry and Biology,Beijing University of Technology,Beijing100124,China [2]Lanzhou University Second Hospital,Lanzhou 730030,China [3]Minzu University of China,Beijing 100081,China

出　　处：《Chinese Chemical Letters》2021年第11期3446-3449,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.21625501,21936001);the Beijing Outstanding Young Scientist Program(No.BJJWZYJH01201910005017).

摘　　要：Single-cell imaging,a powerful analytical method to study single-cell behavior,such as gene expression and protein profiling,provides an essential basis for modern medical diagnosis.The coding and localization function of microfluidic chips has been developed and applied in living single-cell imaging in recent years.Simultaneously,chip-based living single-cell imaging is also limited by complicated trapping steps,low cell utilization,and difficult high-resolution imaging.To solve these problems,an ultra-thin temperature-controllable microwell array chip(UTCMA chip)was designed to develop a living single-cell workstation in this study for continuous on-chip culture and real-time high-resolution imaging of living single cells.The chip-based on ultra-thin ITO glass is highly matched with an inverted microscope(or confocal microscope)with a high magnification objective(100×oil lens),and the temperature of the chip can be controlled by combining it with a home-made temperature control device.High-throughput single-cell patterning is realized in one step when the microwell array on the chip uses hydrophilic glass as the substrate and hydrophobic SU-8 photoresist as the wall.The cell utilization rate,single-cell capture rate,and microwell occupancy rate are all close to 100%in the microwell array.This method will be useful in rare single-cell research,extending its application in the biological and medical-related fields,such as early diagnosis of disease,personalized therapy,and research-based on single-cell analysis.

关 键 词：Ultra-thin microchip Temperature controllable Living single cells One-step patterning Continuous culture High-resolution imaging 

分 类 号：Q2-33[生物学—细胞生物学]