玉米穗发芽突变体vp-like4的转录组分析  

Transcriptome Analysis of Maize Viviparous Mutant vp-like4

作　　者：孙明昊 郭宇航 李健 郑军 杨伟光[1] SUN Minghao;GUO Yuhang;LI Jian;ZHENG Jun;YANG Weiguang(College of Agronomy,Jilin Agricultural University,Changchun 130118,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;College of Agronomy,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]吉林农业大学农学院,长春130118 [2]中国农业科学院作物科学研究所,北京100081 [3]山西农业大学农学院,太谷030801

出　　处：《吉林农业大学学报》2021年第6期642-650,共9页Journal of Jilin Agricultural University

基　　金：国家重点研发计划项目(2016YFD0101002)。

摘　　要：穗发芽是威胁粮食安全的全球性重要灾害。以玉米穗发芽突变体(viviparous)vp-like4与自交系郑58、Mo17组配的F;分离群体为材料,利用转录组测序技术(RNA-seq),结合生物信息学方法,比较野生型和与之对应的突变体的差异表达基因,并分析差异基因功能以及相关的代谢通路,探索调控玉米穗发芽发生的分子机制。结果表明:在突变体中共有3376个差异表达基因(DEGs),其中有2252个上调表达基因,1124个下调表达基因。GO(Gene ontology)功能富集分析结果显示,2151个差异基因被注释,并被富集在716个GO分类条目上,这些被富集的条目主要与新陈代谢以及胁迫响应相关。参考KEGG数据库对差异基因的代谢途径进行分析,发现差异基因显著富集在植物激素信号转导(Plant hormone signal transduction)、淀粉和蔗糖代谢(Starch and sucrose metabolism)、光合作用(Photosynthesis)等途径中。以上结果表明,筛选到的差异基因以及其所属的GO分类条目和代谢途径与玉米穗发芽的发生紧密相关,同时也为深入研究穗发芽基因以及相关的调控网络提供了重要的数据支持。Viviparity is one of the major disasters threatening food security.To explore the molecular mechanism of regulating maize viviparity,F;segregating population derived from the hybrid of maize viviparous vp-like 4 with inbred lines Zheng58 and Mo17 was used as materials,the differentially expressed genes between wild-type and corresponding mutants were compared by transcriptome sequencing(RNA SEQ)and bioinformatics,and the functions of the differentially expressed genes and related metabolic pathways were analyzed.The results showed that there were 3376 differentially expressed genes(DEGS)in the mutant,of which 2252 were up-regulated and 1124 were down regulated.GO(Gene ontology)functional enrichment analysis indicated that 2151 DEGs were annotated and enriched in 716 GO taxonomic entries,which were mainly correlated to metabolism and stress response.Referring to KEGG database,the metabolic pathways of differential genes were analyzed.It was found that differential genes were significantly enriched in plant hormone signal transduction,starch and sucrose metabolism,photosynthesis and other pathways.The above results show that DEGs,GO taxonomic entries and metabolic pathways were closely related to viviparity of maize.These results will provide important data supports for investigating viviparous genes and related regulatory network comprehensively.

关 键 词：玉米 穗发芽 vp-like4 vp15 转录组测序 差异表达基因 

分 类 号：S513[农业科学—作物学]