玉米抗冷相关基因ZmSAMDC克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of ZmSAMDC Gene Related to Maize Cold Resistance

作　　者：金时酉 刘畅 焦鹏 费建博 马义勇[2] 关淑艳[2] JIN Shiyou;LIU Chang;JIAO Peng;FEI Jianbo;MA Yiyong;GUAN Shuyan(College of Life Sciences,Jilin Agricultural University,Changchun 130118,China;College of Agronomy,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学生命科学学院,长春130118 [2]吉林农业大学农学院,长春130118

出　　处：《吉林农业大学学报》2021年第6期651-656,共6页Journal of Jilin Agricultural University

基　　金：吉林省教育厅科学技术项目(JJKH20210346KJ,JJKH20210351KJ);吉林省科技支撑计划项目(20200402023NC)。

摘　　要：在水稻中同源比对得到玉米的SAMDC基因,并利用生物信息学在线软件,对该基因编码的蛋白质结构及理化性质等进行分析。结果表明:ZmSAMDC为疏水性非跨膜类蛋白,无信号肽结构。ZmSAMDC蛋白分子量大小为43283.12 D,等电点为4.78,氨基酸数共398个,蛋白质不稳定系数为38.32,预测该蛋白为稳定蛋白。ZmSAMDC蛋白存在44个潜在磷酸化位点,其中23个丝氨酸(Ser)磷酸化位点,12个苏氨酸(Thr)磷酸化位点,9个酪氨酸(Tyr)磷酸化位点,磷酸化主要集中在苏氨酸、丝氨酸、酪氨酸残基上。ZmSAMDC蛋白的二级结构以α-螺旋、延伸链、无规则卷曲及β-转角组成。ZmSAMDC结构域为SAM_ecarbox结构域,亚细胞主要定位在胞外基质上。In this experiment,the SAMDC gene in maize was obtained by homologous alignment in rice,and the protein structure and physical and chemical properties of the gene were analyzed by bioinformatics software.The results showed that ZmSAMDC is a hydrophobic non-transmembrane protein with no signal peptide structure.The molecular weight of ZmSAMDC protein is 43283.12 D,the isoelectric point is 4.78,and the number of amino acids is 398.The half-life in E.coli is predicted to be greater than 10 hours,and the protein instability coefficient is 38.32.The protein is a stable protein.ZmSAMDC protein has 44 potential phosphorylation sites,including 23 serine(Ser)phosphorylation sites,12 threonine(Thr)phosphorylation sites,9 proline(Tyr)phosphorylation sites,and phosphoric acid mainly concentrated on threonine,serine and proline residues.The secondary structure of ZmSAMDC protein consists ofα-helix,extended chain,random coil andβ-turn.The ZmSAMDC domain is the SAM_ecarbox domain,and the subcellular cells are mainly localized on the extracellular matrix.This study provides new information for the study of the structure and function of the new maize gene,SAMDC,and lays a foundation for the study of ZmSAMDC gene.

关 键 词：玉米 SAMDC 克隆 生物信息学 结构预测 

分 类 号：S513[农业科学—作物学]