壳聚糖/泊洛沙姆复合温敏水凝胶的制备  

Preparation of Chitosan/Pluronic Composite Thermo-Sensitive Hydrogel

作　　者：冯天琪 王春丽[1] 王思瑶 吴鸿艳 王兆国[1] 郝林琳[1] FENG Tianqi;WANG Chunli;WANG Siyao;WU Hongyan;WANG Zhaoguo;HAO Linlin(College of Animal Science,Jilin University,Changchun 130062,China)

机构地区：[1]吉林大学动物科学学院,长春130062

出　　处：《吉林农业大学学报》2021年第6期679-684,共6页Journal of Jilin Agricultural University

基　　金：吉林省科技发展计划项目(20190303040SF)。

摘　　要：泊洛沙姆是一种经典的温敏型共聚物,随着温度的变化产生溶胶-凝胶的相转变,但水凝胶结构不稳定,难以长期保存,并在创伤敷料应用上功能单一。以泊洛沙姆F127和F68为基体,添加壳聚糖作为抑菌材料,制备一种可抑菌的温度响应型原位水凝胶(F127/F68/CS),可应用于不规则伤口愈合。通过试管倒置法、扫描电子显微镜(SEM)、平板涂布法以及对伤口的贴合程度对F127/F68/CS复合水凝胶的温敏性、微观形态、抑菌特性和伤口的贴合度进行了表征。结果表明:F127/F68/CS具有温敏性,在体温下形成水凝胶;在水凝胶断面SEM测试中发现,复合水凝胶内部疏松多孔,呈现出三维网状结构;抑菌试验发现,CS的加入增加了体系的抑菌特性,且抑菌效果明显;伤口贴合度试验显示,水凝胶可以紧密贴合伤口。F127/F68/CS复合水凝胶有望在减少伤口感染,促进创伤愈合的治疗方面得以应用。Pluronic is a classical thermo-sensitive polymer that achieves sol-gel transition with the change of temperature.However,the structure of hydrogel is unstable,and it is difficult to preserve long,and has a single function in wound dressing application.In this research,the thermo-sensitive composite hydrogel was synthesized by mixing pluronic F127 and F68 with an antibacterial component CS which can also be used for irregular wound healing.The temperature sensitivity,microscopic morphology,antibacterial activity and the fit to the wound were investigated by test tube inversion,SEM,bacterial coating method and wound model.The results showed that the composite hydrogel was thermo-sensitive,and formed gelatin at body temperature.The F127/F68/CS hydrogel showed a structure feature of high porosity and 3-dimensional networks.The antibacterial was gradually increased with the increase of CS.And the hydrogel could adhere tightly on the wound.F127/F68/CS composite hydrogel is expected to reduce wound infection and promote wound healing due to its convenience of preparation,temperature sensitivity,suitable porous structure,antibacterial properties and good wound adhesion.

关 键 词：壳聚糖 泊洛沙姆 温敏特性 复合水凝胶 

分 类 号：R318.08[医药卫生—生物医学工程]