谷氨酸棒菌DC1对重金属污染土壤的低温修复及其稳定性  

Low Temperature Remediation of Heavy Metal Contaminated Soil by Corynebacterium glutamicum DC1 and Its Stability

作　　者：丁乙航 HoaiThuong Do 赵子郡 李想 龙振华 李明堂[1] DING Yihang;HoaiThuong Do;ZHAO Zijun;LI Xiang;LONG Zhenhua;LI Mingtang(College of Resources and Environment,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学资源与环境学院,长春130118

出　　处：《吉林农业大学学报》2021年第6期713-720,共8页Journal of Jilin Agricultural University

基　　金：国家自然科学基金项目(41877136)。

摘　　要：耐冷菌在低温下能通过诱导作用形成重金属的碳酸盐沉淀,在寒区重金属污染土壤修复方面具有重要的应用潜力,但目前缺乏对多种重金属污染土壤的修复及其稳定性的研究。试验研究了1株耐冷碳酸盐矿化菌(谷氨酸棒菌DC1)在低温条件下对土壤中4种重金属的固定作用及其稳定性。结果表明:菌株DC1的发酵液可通过诱导作用将土壤中的可交换态Cd、Pb、Cu、Ni转化成碳酸盐结合态,转化比例分别为79.8%、72.6%、39.9%、33.6%,并提高土壤的pH,从而使土壤中有效态Cd、Pb、Cu、Ni的含量分别降低了90.5%、83.4%、35.1%、36.6%。土壤酸化可降低菌株DC1对土壤中Cu和Ni的固定效果,但对Pb和Cd无明显影响。模拟旱田、水田和施肥培养条件下,菌株DC1诱导的Pb和Cd碳酸盐沉淀在土壤中的重金属释放能力较低,培养30 d后土壤有效态Pb和Cd含量均无明显增加。说明菌株DC1可用于重金属污染土壤的碳酸盐沉淀修复,在Pb和Cd污染土壤修复方面有明显的应用优势,但对于Cu和Ni污染土壤的修复需要注意土壤酸化对修复效果的影响。Psychrotolerant bacteria,which can induce carbonate precipitation of heavy metals at low temperature,have important application potential in the remediation of heavy metal contaminated soil in cold area.However,there is a lack of research on remediation and stability of various heavy metal contaminated soil.In this study,the immobilization of four heavy metals by a psychrotolerant carbonate mineralizing bacteria(Corynebacterium glutamicum DC1)and its stability in soil were studied.The results showed that the bacterial culture of strain DC1 could transform exchangeable Cd,Pb,Cu and Ni into carbonate bound through inducing carbonate precipitation with the conversion ratio of 79.8%,72.6%,39.9%and 33.6%,respectively in soil,resulting in the reduction of te available Cd,Pb,Cu and Ni by 90.5%,83.4%,35.1%and 36.6%,respectively,together with the increase of soil pH.Soil acidification decreased the stability of Cu and Ni by strain DC1,but there was no significant effect on Pb and Cd.Under the conditions of simulated dry and paddy soil and fertilizer application,there was no significant release of Pb and Cd in carbonate precipitates induced by strain DC1 in the soil,and the soil available Pb and Cd were not significantly increased after 30 days of incubation.The above results indicate that strain DC1 could be used for remediation of heavy metal contaminated soil through inducing carbonate precipitate,especially for Pb and Cd contaminated soil.However,for Cu and Ni,more attention should be paid to soil acidification.

关 键 词：谷氨酸棒菌 耐冷菌 土壤重金属污染 碳酸盐沉淀 低温修复 稳定性 

分 类 号：S156[农业科学—土壤学]