2株猪源乳酸杆菌的抗逆性和生长特性分析  

Stress Resistance and Growth Characteristics of Two Strains from Swine Lactobacillus

作　　者：靳胜男 舒慧萍 张冬星[1] 康元环[1] 单晓枫[1] 钱爱东[1] JIN Shengnan;SHU Huiping;ZHANG Dongxing;KANG Yuanhuan;SHAN Xiaofeng;QIAN Aidong(College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学动物科学技术学院,长春130118

出　　处：《吉林农业大学学报》2021年第6期734-740,共7页Journal of Jilin Agricultural University

基　　金：国家自然科学基金项目(31201927);吉林省教育厅“十三五”科学技术项目(JJKH20210367KJ);吉林省科技厅自然科学基金学科布局项目(20170101016JC);吉林农业大学博士启动基金项目(201801)。

摘　　要：为分析猪源嗜淀粉乳杆菌L-102(Lactobacillus amylovorus)和约氏乳杆菌L-76(Lactobacillus johnsonii)的抗逆性和生长特性,为乳酸菌进一步的体外研究提供理论依据。分别对L-102、L-76乳酸杆菌的耐酸碱、耐胆盐、耐胰蛋白酶、黏附性、抑菌性、产酸能力、生长能力及对小鼠致病性进行了测定和分析。结果表明:乳酸杆菌L-102和L-76对pH 2~11的酸碱环境具有抵抗能力,可耐受生长介质中含0.1%~0.2%的胆盐和1%的胰蛋白酶;对IPEC-J2细胞有一定的黏附作用;能抑制大肠杆菌、沙门氏菌和金黄色葡萄球菌的生长;L-102和L-76乳酸杆菌的产酸在pH 4.1~4.3;培养4 h后进入生长对数期;经50℃和60℃高温作用5~15 min,其活菌数可达到10^(7)CFU/mL;通过腹腔注射和灌胃小鼠发现,小鼠无死亡。表明嗜淀粉乳杆菌L-102和约氏乳杆菌L-76具有潜在的益生菌特性,有望发展成为用于养猪行业的饲用益生乳酸菌。The purpose of this study is to analyze and investigate stress resistance and growth characteristics of Lactobacillus amylovorus L-102 and Lactobacillus johnsonii L-76 from swine, and to provide a theoretical basis for further in vitro studies of lactic acid bacteria. Acid-base tolerance, bile tolerance, trypsin tolerance, adhesiveness, bacteriostatic ability, acid production ability, growth curve and pathogenicity of Lactobacillus L-102 and L-76 were measured and analyzed. The results showed that Lactobacillus L-102 and L-76 exhibited resistance to acid-base environment with pH values of 2-11, and could tolerate 0.1%-0.2% bile salts and 1% trypsin in growth medium. The two Lactobacillus strains had certain adhesion to IPEC-J2 cell and inhibited the growth of Escherichia coli, Salmonella Typhimurium and Staphylococcus aureus. Moreover, the range of acid production of Lactobacillus L-102 and L-76 is 4.10-4.30. After 4 hours of culture, it entered the logarithmic phase of growth. The viable count achieved 10^(7)CFU/mL when Lactobacillus L-102 and L-76 were sustained at 50 ℃ and 60 ℃ for 5-15 min. No death mice were found in mice injected intraperitoneally and administered intragastrically with Lactobacillus L-102 and L-76. Data indicated that Lactobacillus amylovorus L-102 and Lactobacillus johnsonii L-76 have potential probiotic characteristics and could develop into feeding probiotic lactic acid bacteria for swine industry.

关 键 词：猪 嗜淀粉乳杆菌 约氏乳杆菌 抗逆性 生物学特性 

分 类 号：S858.28[农业科学—临床兽医学]