一测多评法同时测定葆肾合剂中5种成分的含量  

Simultaneous determination of five ingredients in Baoshen mistura by quantitative analysis of multi-components by single marker

作　　者：冯子芳[1] 费逸明 袁晓航[1] 陈晓伟 胡敏敏[1] 刘丽萍 FENG Zifang;FEI Yiming;YUAN Xiaohang;CHEN Xiaowei;HU Minmin;LIU Liping(Wuxi Traditional Chinese Medicine Hospital Affiliated to Nanjing University of Chinese Medicine,Jiangsu,Wuxi 214000,China;Jiangsu Vocational College of Medicine,Jiangsu,Yancheng 224005,China)

机构地区：[1]南京中医药大学附属无锡市中医医院,江苏无锡214000 [2]江苏医药职业学院,江苏盐城224005

出　　处：《中国医药科学》2022年第1期51-54,132,共5页China Medicine And Pharmacy

基　　金：江苏省无锡市卫生和计划生育委员会中医药科研项目(ZYZL201802);江苏省卫生健康委科研项目(M2020104)。

摘　　要：目的建立一测多评(QAMS)法同时测定葆肾合剂中绿原酸、咖啡酸、薏苡素、鞣花酸和毛蕊异黄酮葡萄糖苷5种成分的含量。方法选取Waters XBridge C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈(A)-0.2%磷酸水溶液(B),梯度洗脱。以绿原酸为内参物,建立相对校正因子,计算其含量,比较外标法与QAMS法的差异性。结果绿原酸、咖啡酸、薏苡素、鞣花酸和毛蕊异黄酮葡萄糖苷在范围内线性关系良好(r≥0.9996),不同实验条件下相对校正因子重现性良好,一测多评法与外标法测得值之间无显著性差异(RAD<2%)。结论建立的一测多评法准确、重复性好,可用于葆肾合剂中5种活性成分的同时测定,实现质量控制。Objective To establish a quantitative analysis of multi-components by single marker(QAMS method for the simultaneous determination of chlorogenic acid,caffeic acid,coixol,ellagic acid and mullein isoflavone glucoside in Baoshen mistura.Methods A Waters XBridge C18 column(250 mm×4.6 mm,5μm)was selected with the mobile phase of acetonitrile(A)-0.2%phosphoric acid water(B),and the gradient elution was performed.The chlorogenic acid was used as the internal reference,and the relative correction factors were set for the calculation of the content of chlorogenic acid,and the differences between the external standard method and QAMS method were compared.Results The chlorogenic acid,caffeic acid,coixol,ellagic acid and mullein isoflavone glucoside showed good linearity within a certain range(r≥0.9996).There was a good reproducibility in the relative correction factors under different experimental conditions,and there was no significant difference in the values measured by the QAMS method and the external standard method(RAD<2%).Conclusion The established QAMS method is accurate with good reproducibility,which can be used for the simultaneous determination of the five active ingredients in Baoshen mistura to achieve quality control.

关 键 词：一测多评法 葆肾合剂 相对校正因子 质量控制 

分 类 号：R286.0[医药卫生—中药学]