基于网络药理学探讨肉苁蓉-巴戟天药对治疗股骨头坏死的作用机制  

Mechanism of“Herba Cistanches-Radix Morindae Officinalis”in the Treatment of Osteonecrosis of the Femoral Head Based on Network Pharmacology

作　　者：郭雪峰[1,2] 侯德才 于睿[1] Guo Xuefeng;Hou Decai;Yu Rui(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;Shenyang Orthopedic Hospital,Shenyang 110041,China;The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China)

机构地区：[1]辽宁中医药大学,沈阳110847 [2]沈阳市骨科医院,沈阳110041 [3]辽宁中医药大学附属医院,沈阳110032

出　　处：《世界科学技术-中医药现代化》2021年第10期3749-3756,共8页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：2020年辽宁省教育厅科学研究经费项目(L202043):基于PI3K/AKT/mTOR信号通路探究“从肾论治”激素性股骨头坏死的作用机制,负责人:郭雪峰。

摘　　要：目的基于网络药理学方法探讨肉苁蓉-巴戟天药治疗股骨头坏死的作用机制。方法通过中药系统药理学数据库与分析平台(TCMSP)对肉苁蓉-巴戟天药对中活性成分进行筛选,通过GeneCards、OMIM数据库检索股骨头坏死疾病靶点,取得交集基因后使用Cytoscape 3.7.1建立化合物-疾病-靶点调控网络,基于生物学信息注释数据库(DAVID)对核心靶点进行基因本体(GO)功能富集分析与基因组百科全书(KEGG)通路富集分析。结果共得到肉苁蓉-巴戟天药对的21个活性成分和182个潜在靶点,相关的疾病靶点727个,GO富集分析主要包括细胞凋亡、脂质代谢异常、活性氧反应等功能途径;KEGG通路分析主要包括缺氧诱导因子-1信号通路、p53信号通路、肿瘤坏死因子信号通路等。结论基于网络药理学初步揭示出肉苁蓉-巴戟天药对治疗股骨头坏死多成分、多靶点、多通路的整体调节作用,其作用机制可能与脂质代谢异常、细胞凋亡、缺氧诱导因子-1信号通路、p53信号通路密切相关。Objective To discover the mechanism of"Herba Cistanches-Radix Morindae Officinalis"in the treatment of osteonecrosis of the femoral head based on network pharmacology.Methods TCMSP was used for screening effective components and targets of"Herba Cistanches-Radix Morindae Officinalis".GeneCards and OMIM were used to screen targets of osteonecrosis of the femoral head.Cytoscape 3.7.1 was used to build component-disease-target network,and analyze GO and KEGG enrichment.Results 21 effective components and 182 potential targets of"Herba CistanchesRadix Morindae Officinalis"were obtained based on TCMSP.727 targets related to the occurrence and development of osteonecrosis of the femoral head were obtained.GO function enrichment analysis included apoptosis,response to lipid,response to reactive oxygen.KEGG pathway enrichment analysis included p53 signaling pathway,HIF-1 signaling pathway,p53 signaling pathway.Conclusion Based on network pharmacology,it preliminarily reveals the overall regulatory effect of"Herba Cistanches-Radix Morindae Officinalis"on multi-component,multi-target and multichannel treatment of femoral head necrosis.Its mechanism may be closely related to abnormal lipid metabolism,apoptosis,HIF-1 signaling pathway,p53 signaling pathway.

关 键 词：网络药理学 股骨头坏死 肉苁蓉 巴戟天 

分 类 号：R285[医药卫生—中药学]