含偶氮苯的光致形变液晶聚合物的细胞生物相容性  

Cell Biocompatibility of Photodeformable Azobenzene-containing Liquid Crystal Polymers

作　　者：骆沙曼 孙浩哲 颜世强 黄晖[1,3] 张炜佳 韦嘉[2] 俞燕蕾 LUO Sha-Man;SUN Hao-Zhe;YAN Shi-Qiang;HUANG Hui;ZHANG Wei-Jia;WEI Jia;YU Yan-Lei(Institutes of Biomedical Sciences,Fudan University,Shanghai 200433,China;Department of Materials Science,Fudan University,Shanghai 200433,China;State Key Laboratory of Molecular Engineering of Polymers,Fudan University,Shanghai 200433,China)

机构地区：[1]复旦大学生物医学研究院,上海200433 [2]复旦大学材料科学系,上海200433 [3]复旦大学聚合物分子工程国家重点实验室,上海200433

出　　处：《应用化学》2021年第10期1371-1381,共11页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(Nos.51927805,21734003);中国博士后科学基金(No.2019M651382)资助。

摘　　要：对一种含偶氮苯的光致形变液晶聚合物(PC11AB6)的细胞生物相容性进行了研究,并与其它3种常见生物应用材料(聚苯乙烯、玻璃、聚二甲基硅氧烷)进行了对比。结果表明,经过亲水处理后,4种材料上的Madin-Darby狗肾(MDCK)细胞、人脐静脉内皮细胞(HUVEC)和诱导性多能干细胞(iPSC)的铺展状态无明显差异;在光响应聚合物PC11AB6薄膜上培养的HUVEC中内皮细胞标志物VWF的表达和iPSC中干细胞标志物SOX2和OCT3的表达与其它3种材料相比无显著差异;HUVEC在PC11AB6薄膜上的生长增殖情况与在聚苯乙烯或玻璃上相当,且明显优于聚二甲基硅氧烷。因此,含偶氮苯的光致形变液晶聚合物PC11AB6具备细胞生物相容性,在可控微流体传输、微量生化分析和芯片实验室等领域具有一定的应用前景。The cell biocompatibility of photodeformable azobenzene-containing liquid crystal polymer(PC11AB6)was investigated and compared with other common materials(polystyrene,glass and polydimethylsiloxane)in this study.After modifying surfaces with plasma treatment and coating,there was no significant difference in spreading state of Madin-Darby canine kidney(MDCK)cells,human umbilical vein endothelial cells(HUVEC)and induced pluripotent stem cells(iPSC)on the four materials;The expression of endothelial cell marker VWF in HUVEC and the expression of stem cell markers SOX2 and OCT3 in iPSC cultured on polymer PC11AB6 film were not significantly different from those of the other three materials;The growth and proliferation of HUVEC on the PC11AB6 film were comparable to those on polystyrene or glass,and were significantly better than those of polydimethylsiloxane.Overall,photodeformable azobenzene-containing LCP PC11AB6 is cell biocompatible,and shows potential in controllable microfluid transportation,micro biochemical analysis and the laboratory-on-chip system.

关 键 词：光致形变液晶聚合物 偶氮苯 细胞生物相容性 

分 类 号：O631[理学—高分子化学]