油茶炭疽病病原菌的分离与鉴定  

Pathogen Isolation and Identification of Camellia oleifera Anthracnose

作　　者：陈美霞 张承康 彭成彬 王泽榕 石玲 阮俊峰 郑世仲 刘伟 CHEN Meixia;ZHANG Chengkang;PENG Chengbin;WANG Zerong;SHI Ling;LIU Ting;RUAN Junfeng;ZHENG Shizong;LIU Wei(Ningde Normal University,College of Life Sciences,Ningde 352100,China;Industry and University Research Cooperation Demonstration Base in Fujian Province,Ningde 352100,China;Fujian Agriculture and Forestry Uni-versity,College of Agriculture,Fuzhou 350002,China;Ningde Forestry Bureau,Ningde 352100,China)

机构地区：[1]宁德师范学院生命科学学院,福建宁德352100 [2]科技厅产学研合作示范基地,福建宁德352100 [3]福建农林大学农学院,福建福州350002 [4]宁德市林业局,福建宁德352100

出　　处：《安徽农学通报》2022年第3期102-105,175,共5页Anhui Agricultural Science Bulletin

基　　金：福建省林业科技项目(闽林科便函[2019]16号);福建省教育厅新世纪优秀人才项目(2018-47号);宁德师范学院校级项目(2020Z01,2019T02,2018ZX405)。

摘　　要：目的:油茶炭疽病是一种重要病害,引起油茶树落果和落蕾,落果率在20%~40%,严重时可达80%以上,给茶农带来的经济损失较大。为明确引起该病害的主要病原菌,特进行了病原菌的分离与鉴定。方法:以油茶病叶作为材料,根据科赫法则采用离体组织分离法进行分离,采用形态学结合ITS、ACT、CAL多基因系统分析鉴定。结果:从病叶中分离得到5株病原菌株DH1、DH2、DH3、DH4、DH5,根据病原菌株的形态观察和致病性测定,鉴定为炭疽菌属。PDA培养基上分别挑取菌丝体并提取基因组DNA,进行ITS、ACT、CAL PCR多基因序列联合分析,MEGA 7.0构建系统发育树,结果显示,5个菌株均为炭疽属,其中DH1、DH2、DH4、DH5为果生炭疽菌,DH3为山茶炭疽菌,形态学鉴定与分子生物学对病原菌的鉴定结果一致。结论:引起油茶炭疽病的主要病原菌为果生炭疽菌和山茶炭疽菌。Objective:Camellia oleifera anthracnose is major disease in the producing areas,which can generally cause fruit drop and bud drop.The fruit drop rate is within 20%to 40%,seriously more than 80%,causing great eco⁃nomic losses to the farmers.In order to confirm the infectious pathogen,the characteristics of the isolated pathogen were studied and identified.Method:We obtain leaves infected by C.oleifera anthracnose as research materials,iso⁃late pathogenic bacteria according to Koch,s postulates,then identified by morphological method and phylogenetic analysis based on ITS、ACT、CAL.Result:5 pathogen strains DH1、DH2、DH3、DH4、DH5 were identified based on cultural characteristics and pathogenecity of the isolates.DNA mycelium was extracted from PDA medium,ITS、ACT、CAL were amplified respectively after passing the test.The phylogenetic tree was constructed by mega7.0.The results showed that DH1、DH2、DH4、DH5 were Colletotrichum fructicola,DH3 was C.camelliae,which proved that the morphological identification was consistent with the molecular biological identification of pathogenic bacteria.Conclusion:The major pathogen were C.fructicola and C.camelliae.

关 键 词：油茶 炭疽病 病原菌分离鉴定 多基因序列分析 

分 类 号：S435[农业科学—农业昆虫与害虫防治]