miR-430对斑马鱼原始生殖细胞迁移和性腺发育影响的初步研究  

Preliminary Functional Analysis of miR-430 in Regulating Zebrafish(Danio rerio)Primordial Germ Cell Migration and Gonad Development

作　　者：林小涵 金超凡 高晨 汪波[1] 贺艳[1,2] 齐洁 张全启[1,2] Lin Xiaohan;Jin Chaofan;Gao Chen;Wang Bo;He Yan;Qi Jie;Zhang Quanqi(Key Laboratory of Marine Genetics and Breeding Ministry of Education, Ocean University of China, Qingdao 266003, China;Laboratary for Marine Fisheries Science and Food Production Process, Pilot National Laboratary for Marine Science and Technology (Qingdao), Qingdao 266237, China)

机构地区：[1]中国海洋大学海洋生物遗传学与育种教育部重点实验室,山东青岛266003 [2]青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室,山东青岛266237

出　　处：《中国海洋大学学报（自然科学版）》2022年第3期72-79,共8页Periodical of Ocean University of China

基　　金：国家重点研究发展计划项目(2018YFD0901205)资助。

摘　　要：为了探究microRNAs(miRNAs)在斑马鱼生殖细胞发育过程中所起的作用,本研究在斑马鱼胚胎期过表达miR-430,结果发现原始生殖细胞(Primordial germ cells,PGCs)迁移出现紊乱,并且通过qRT-PCR发现nanos3、piwil2及tdrd7等生殖质基因的表达水平显著下调。此外,在斑马鱼性腺发育过程中,通过组织学观察发现过表达miR-430会导致雌性斑马鱼卵母细胞发育异常、卵巢发育迟缓,但是对雄性生殖细胞及性腺的发育没有造成明显的影响,并且进一步统计分析表明,miR-430不影响斑马鱼的性别分化。综上所述,本研究初步明确了过表达miR-430对斑马鱼PGCs迁移、性腺发育以及性别分化的影响,并为进一步探究miR-430在斑马鱼生殖细胞发育过程中的功能及分子机制奠定了理论基础。To explore the role of microRNAs(miRNAs)in the development of zebrafish germ cells,this study overexpressed miR-430 during the embryonic stage of zebrafish and found that the migration of PGCs was aberrant.qRT-PCR results demonstrated that the expression of germplasm genes,such as nanos3,piwil2,and tdrd7 was significantly down-regulated.In addition,in the process of gonadal development in zebrafish,histological observation showed that the overexpression of miR-430 would lead to abnormal oocyte development and retarded ovaries development in female zebrafish,but had no significant effect on the development of male germ cells and testis.Further statistical analysis demonstrated that the overexpression of miR-430 did not affect the sex differentiation of zebrafish.In summary,our research preliminarily determined the effect of miR-430 on PGCs migration,gonad development,and sex differentiation,laying a theoretical foundation for further exploration of the function and molecular mechanism of miR-430 in the development of zebrafish germ cells.

关 键 词：斑马鱼 miR-430 显微注射 PGCS 卵母细胞 性腺发育 

分 类 号：Q38[生物学—遗传学]