振动刺激诱发前庭诱发电位检测系统的初步研究  

A Preliminary Study on a Vibration-Iduced Vestibular Sensory-Evoked Potential Test System

作　　者：高滢[1] 张青 鸭頭辉 山岨達也 岩崎真一 张滟[1] 马伟军[1] 陈耔辰 张玉忠[1] 许珉[1] 任晓勇[1] GAO Ying;ZHANG Qing;Kamogashira Teru;Yamasoba Tatsuya;Iwasaki Shinichi;ZHANG Yan;MA Weijun;CHEN Zichen;ZHANG Yuzhong;XU Min;REN Xiaoyong(Department of Otolaryngology Head and Neck Surgery,Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an,710004,China;Department of Otolaryngology Head and Neck Surgery,Faculty of Medicine University of Tokyo,Tokyo,1130034,Japan;Department of Otolaryngology&Head and Neck Surgery,Xinhua Hospital,Shanghai Jiaotong University School of Medi‐cine,Ear Institute,Shanghai Jiaotong University School of Medicine,Shanghai Key Laboratory of Translation Medicine on Ear and Nose Diseases,Shanghai,200092,China;Department of Otolaryngology,Head and Neck Surgery,Graduate School of Medical Sciences,Nagoya City University,Nagoya,467-8601,Japan)

机构地区：[1]西安交通大学第二附属医院耳鼻咽喉头颈外科 [2]日本东京大学耳鼻咽喉头颈外科 [3]上海交通大学医学院附属新华医院耳鼻咽喉头颈外科,上海交通大学医学院耳科学研究所,上海市耳鼻疾病转化医学重点实验室 [4]日本名古屋市立大学耳鼻咽喉头颈外科

出　　处：《中华耳科学杂志》2022年第1期111-115,共5页Chinese Journal of Otology

基　　金：国家自然科学基金项目(82101224,81970891,81670945);陕西省重点研发计划项目(2019SF-182);陕西省国际合作重点项目(2020KWZ-19);院基金自由探索项目(2020YJ(ZYTS)043)。

摘　　要：目的探索振动刺激诱发前庭诱发电位(VsEP Vestibular Evoked Potential)检测系统的构建及电位记录方法。旨在建立一套操作简便,无创伤,可长期反复操作,结果稳定的前庭诱发电位检测系统。方法选取健康小鼠6只,随机分为健康对照组(3只)和庆大霉素造模组(3只)。使用振动刺激诱发记录前庭诱发电位(Vestibular evoked potential,VsEP),并与声刺激诱发的听性脑干反应(ABR)进行比较。结果6只小鼠均能引出稳定的VsEP波。庆大霉素造模组在SPLR波形振动刺激下比较健康对照组阈值升高(P<0.01),N1-P1振幅降低(P<0.05)。ABR阈值无明显差异。结论振动刺激诱发的前庭诱发电位检测系统是一种操作简便、无创伤、波形稳定的动物前庭功能检测系统,可用于小鼠前庭功能电生理状态的客观评价研究。Objective To develop a testing system and protocols for vibration elicited vestibular sensory-evoked potentials(VsEPs)that are non-invasive,stable and easy to use with reproducible results for animal studies.Method Six healthy mice were selected to be in a gentamicin treatment or control group(n=3 in each group)and received VsEP and click elicited ABR tests.Result Stable VsEP waves could be elicited in all 6 mice with typical waves,although with significantly higher thresholds and lower amplitudes in the gentamicin group than in the control group under SPLR stimulation(P<0.01 and P<0.05,respectively).Conclusion A vibration-elicited VsEP test system is easy to set up and non-invasive,and can produce repeatable and stable waveforms reflecting vestibular function,which can be widely used in objective vestibular electrophysiological evaluation in mice.

关 键 词：前庭诱发电位 耳石器 小鼠 

分 类 号：R764[医药卫生—耳鼻咽喉科]