差示扫描量热(DSC)图谱匹配识别法在鱼腥草产地快速鉴别中的应用  

Application of differential scanning calorimetry (DSC) in origin identification of Houttuynia cordata

作　　者：邹涛[1] 陈宇迪[1] 莫单丹[2] 赵瑾[1] 和莉颖 郭姝[1] 欧春丽 曾智强 缪剑华[2] Zou Tao;Chen Yudi;Mo Dandan;Zhao Jin;He Liying;Guo Shu;Ou Chunli;Zeng Zhiqiang;Miao Jianhua(Beijing Center for Physical&Chemical Analysis,Beijing 100089,China;Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement,Guangxi Botanical Garden of Medicinal Plant,Nanning 530023,China;NETZSCH Scientific Instruments Trading(Shanghai)Co.,Ltd.,Shanghai 200131,China)

机构地区：[1]北京市理化分析测试中心,北京100089 [2]广西药用植物园,广西药用资源保护与遗传改良重点实验室,南宁530023 [3]耐驰科学仪器商贸(上海)有限公司,上海200131

出　　处：《分析仪器》2022年第1期93-99,共7页Analytical Instrumentation

基　　金：对发展中国家科技援助项目(KY201904001);科技基础资源调查专项(2018FY100700);广西创新驱动发展专项(桂科AA18242040);广西壮族自治区药用植物园广西药用资源保护与遗传改良重点实验室开放性研究项目;北京市理化分析测试中心改革与发展专项(2020Zl0201);北京市科协金桥工程种子资金C类项目;北京市理化分析测试中心2021年改革与发展专项(2021ZL0104)。

摘　　要：中药材的道地性鉴别研究是目前的一个难点。本研究探究了差示扫描量热(DSC)方法在鱼腥草产地鉴别中的应用。通过DSC测量方法的改进,测量参数的优化,得到了稳定、可重复、有辨识度的鱼腥草DSC谱图。研究发现鱼腥草样本在60℃~350℃区间内有两个明显的特征放热峰。不同产地样本的特征反应峰的起始温度、峰值温度、反应焓及DSC曲线的拓扑图形都有明显的差异,而且相同产地样本的DSC曲线具有良好的重复性。在此基础上,本研究将16个不同产地的鱼腥草样本DSC数据建成数据库,引入相似度匹配算法计算得到鱼腥草DSC谱图之间的相似度。通过相似度匹配可以实现鱼腥草产地的高效、精准鉴别,可为中药材产地的鉴别提供新的研究思路和方法。By improving the DSC measurement method and optimizing the measurement parameters, a stable, reproducible and discriminative DSC spectrum of Houttuynia cordata was obtained. It was found that there were two characteristic exothermic peaks in the range of 60℃—350℃ for the Houttuynia cordata samples;the onset temperature, peak temperature, reaction enthalpy and topography of DSC curves of the characteristic peaks of samples from different origins were significantly different;and the DSC curves of samples from the same origin had good reproducibility. On this basis, the DSC spectra of 16 samples from different origins were built into a database, and the similarity matching algorithm was introduced to calculate the similarity between the DSC plots of unknown samples and reference samples. Through similarity matching, efficient and accurate identification of the origin of Chinese herbal medicines can be achieved.

关 键 词：鱼腥草 差示扫描量热 产地鉴别 

分 类 号：R282.5[医药卫生—中药学]