特异性PCR方法鉴别珠芽蓼与其混淆品  

Identification of Polygonum Viviparum and its Adulterants by Specific Polymerase Chain Reaction

作　　者：谢美霞 沈千汇 黄琦 李文佳 李春红 钱正明 Xie Meixia;Shen Qianhui;Huang Qi;Li Wenjia;Li Chunhong;Qian Zhengming(Yichang Shanchengshuidu Cordyceps Co.Ltd,Hubei Yichang 443000,China;Dongguan HEC Cordyceps R&D Co.Ltd.)

机构地区：[1]宜昌山城水都冬虫夏草有限公司,湖北宜昌443000 [2]东莞市东阳光冬虫夏草研发有限公司

出　　处：《中国药师》2022年第1期124-129,共6页China Pharmacist

基　　金：湖北省科技重大专项(编号:2020ACA007);湖北省冬虫夏草工程技术研究中心支持项目(编号:20171007)。

摘　　要：目的:建立一种快速鉴别珠芽蓼与其混淆品的方法。方法:基于珠芽蓼及其混淆品的ITS基因序列,设计特异性引物,通过聚合酶链式反应(PCR)对珠芽蓼及混淆品进行扩增,优化反应体系及条件,并对此方法进行考察。结果:在最佳反应条件下扩增,仅珠芽蓼可扩增出约172 bp的特异性DNA目标条带,12批混淆品均无此条带。结论:本研究首次建立的珠芽蓼特异性PCR方法可快速、准确地鉴别珠芽蓼及其混淆品,具有高度特异性和灵敏度,为中药材珠芽蓼质量评价提供了良好的科学依据。Objective:To establish a rapid molecular identification method for Polygonum viviparum and its adulterants.Methods:Based on the ITS gene sequence of Polygonum viviparum and its adulterants,specific primers were designed to amplify Polygonum viviparum and its adulterants by polymerase chain reaction,and the reaction system and conditions were optimized.Results:Using the optimal reaction conditions,only the template DNA of Polygonum viviparum could be amplified to obtain the specific 172 bp band whereas the diagnostic PCRs of the other 12 batches of adulterants were all negative.Conclusion:The specific PCR method established for the first time can rapidly and accurately identify Polygonum viviparum and its adulterants with high specificity and sensitivity,which provides good scientific basis for the quality evaluation of Polygonum viviparum.

关 键 词：珠芽蓼 特异性引物 聚合酶链式反应 鉴别 

分 类 号：R282.5[医药卫生—中药学]