高胆红素血症对新生大鼠心肌的影响  被引量:1

The effects of hyperbilirubinemia on the myocardium of newborn rats

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作  者:苏敏[1] 王惠颖 任漪 李丽[1] 赵丹丹 黄迪 杨波 高翔羽[1] Su Min;Wang Huiying;Ren Yi;Li Li;Zhao Dandan;Huang Di;Yang Bo;Gao Xiangyu(Department of Neonatology,Xuzhou Central Hospital(The Xuzhou Clinical College of Xuzhou Medical University),Xuzhou 221009,China)

机构地区:[1]徐州市中心医院(徐州医科大学徐州临床学院)新生儿科,221009

出  处:《中华新生儿科杂志(中英文)》2022年第1期70-75,共6页Chinese Journal of Neonatology

基  金:江苏省青年医学重点人才项目(QNRC2016384)。

摘  要:目的探讨不同水平高胆红素血症对新生大鼠心肌的影响。方法选取7日龄新生SD大鼠96只,随机分为对照组(n=32,腹腔注射生理盐水0.5 ml)、实验1组(n=32,腹腔注射胆红素溶液100 mg/kg)和实验2组(n=32,腹腔注射胆红素溶液200 mg/kg)。分别设置0 h、8 h、24 h和48 h 4个时间点,每个时间点8只大鼠。记录大鼠一般情况,测定每个时间点大鼠血清总胆红素(total serum bilirubin,TSB)、心肌肌钙蛋白Ⅰ、心型脂肪酸结合蛋白和B型利钠肽水平;摘取心脏,制作病理切片,镜下观察心肌病理学改变;检测半胱氨酸蛋白酶3(caspase-3)、B细胞淋巴瘤2(B-cell lymphoma-2,bcl-2)和bcl-2相关X蛋白(bcl-2-associated X protein,bax)。应用SPSS 20.0统计软件分析,选用两因素方差分析法。结果在实验1组内和实验2组内,8~48 h TSB[(20.8±3.0~36.5±10.4)μmol/L和(31.9±12.3~67.4±19.0)μmol/L]比0 h[(8.4±2.1)μmol/L和(9.1±2.9)μmol/L]分别升高2.5~4.4倍和3.5~7.4倍。在各时间点3组大鼠心脏组织病理均无明显改变。在48 h时间点,随着TSB水平升高,凋亡相关蛋白caspase-3和bax表达增加,bcl-2表达减少;除实验1组bcl-2灰度值与对照组相似(P=0.255)外,其余各组蛋白灰度值两两比较,差异均有统计学意义(P<0.05)。在实验2组,随着高胆红素血症持续时间延长,caspase-3和bax表达增加,bcl-2表达减少;除8 h亚组bax灰度值与12 h亚组相似(P=0.820)、8 h亚组bcl-2灰度值与0 h亚组相似(P=0.064)外,其余各时间点蛋白灰度值两两比较,差异均有统计学意义(P<0.05)。在8 h、24 h和48 h,实验1组和实验2组心肌肌钙蛋白Ⅰ均高于对照组[(543.5±167.7、675.1±162.4、584.4±125.2)ng/L和(465.7±107.2、769.4±202.1、497.1±100.8)ng/L比(361.7±102.4、324.5±170.5、377.8±65.2)ng/L],差异均有统计学意义(P<0.05),实验1组和实验2组之间差异均无统计学意义(P>0.05)。在各时间点3组间心型脂肪酸结合蛋白和B型利钠肽差异均无统计学意义(P>0.05)。Objective To study the influences of different levels of hyperbilirubinemia on the myocardium of newborn rats.Methods Ninety-six 7-day-old newborn SD rats were selected and randomly assigned into control group(n=32,intraperitoneal injection of normal saline 0.5 ml),test group 1(n=32,intraperitoneal injection of bilirubin solution 100 mg/kg)and test group 2(n=32,intraperitoneal injection of bilirubin solution 200 mg/kg).Four time points were set at 0 h,8 h,24 h and 48 h.The general conditions of 8 rats from every group at each time point were recorded.The total serum bilirubin(TSB),cardiac troponin I(cTnI),heart fatty acid-binding protein(H-FABP)and B-type natriuretic peptide(BNP)were examined.The heart was removed and the pathological changes of the myocardium were observed under microscope.The caspase-3,B-cell lymphoma-2 protein(bcl-2)and bcl-2-associated X protein(bax)were tested.Using SPSS 20.0 statistical software,two-way ANOVA analysis of variance was conducted.Results The TSB in test group 1 and 2 at 8~48 h were 2.5~4.4 times and 3.5~7.4 times higher than at 0 h[(20.8±3.0~36.5±10.4)μmol/L and(31.9±12.3~67.4±19.0)μmol/L vs.(8.4±2.1)μmol/L and(9.1±2.9)μmol/L].No significant changes existed in cardiac histopathology at each time point among the three groups.At 48 h,as TSB level increased,the expression of apoptosis-related proteins caspase-3 and bax increased and the expression of bcl-2 decreased.Significant differences existed in the protein levels between any two groups(all P<0.05),except that bcl-2 in test group 1 was similar to control group(P=0.255).With the prolonged duration of hyperbilirubinemia in test group 2,the expression of caspase-3 and bax increased,while the expression of bcl-2 decreased.Statistically significant differences existed in the protein levels between any two time points(all P<0.05),except that bax in 8 h subgroup was similar to 12 h subgroup(P=0.820),and bcl-2 in 8 h subgroup was similar to 0 h subgroup(P=0.064).The cTnI at 8 h,24 h and 48 h in test group 1 and 2 were all

关 键 词:高胆红素血症 心肌 利钠肽  大鼠 

分 类 号:R722.1[医药卫生—儿科]

 

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