KIF18B 在胃癌细胞增殖、 迁移中的作用及机制研究  

Role of KIF18B in Proliferation and Migration of Gastric Cancer Cells

作　　者：李怡君 王雨[1] 原姗姗[1] 司望利[1] 陈晓露[1] 郭汉青 LI Yijun;WANG Yu;YUAN Shanshan;SI Wangli;CHEN Xiaolu;GUO Hanqing(Department of Gastroenterology,Xi’an Central Hospital,Xi’an,710003,China)

机构地区：[1]西安市中心医院消化科,西安市710003

出　　处：《医学分子生物学杂志》2022年第1期62-67,共6页Journal of Medical Molecular Biology

摘　　要：目的探究驱动蛋白超家族18B(KIF18B)对胃癌细胞增殖、迁移的影响及潜在调控机制。方法采用qRT-PCR和Western印迹分析胃癌组织和细胞(MKN-28、AGS、HGC-27)中KIF18B的表达水平;通过CCK-8、克隆形成以及划痕实验检测胃癌细胞的增殖、迁移情况;Western印迹法检测迁移蛋白(E-cadherin、N-cadherin)和MMP-3的表达。结果KIF18B在胃癌组织和细胞中表达上调;干扰KIF18B显著抑制AGS细胞增殖、集落形成和细胞迁移,提高E-cadherin的蛋白表达,同时降低N-cadherin和MMP-3的蛋白表达;此外,干扰KIF18B能显著抑制CDCA8并促进p53的蛋白表达,过表达CDCA8能逆转KIF18B干扰对细胞增殖、迁移的影响。结论KIF18B通过调控CDCA8/p53信号通路影响胃癌细胞的增殖、迁移。Objective To investigate the role and regulatory mechanismof kinesin family mem-ber 18B(KIF18B)in proliferation and migration of gastric cancer(GC)cells.Methods The expression of KIF18B in GC tissues and cells(MKN-28,AGS and HGC-27)was detected by u-sing qRT-PCR and Western blotting.Cell proliferation was evaluated by CCK-8 and colony formation assays.Wound healing method was used to detect cell migration.The expression levels of E-cadher-in,N-cadherin and MMP-3 were determined by Western blotting.Results The KIF18B expression was up-regulated in GC tissues and cells.Silencing KIF18B dramatically reduced the proliferation,colony formation capability and migration rate of AGS cells.The expression level of E-cadherin was increased,and the N-cadherin and MMP-3 expression levels were decreased.Moreover,the KIF18B knockdown significantly inhibited the CDCA8 expression but promoted the expression of p53 protein.Overexpression of CDCA8 could reverse the effects of KIF18B silencing on cell proliferation and migration.Conclusion KIF18B regulated the proliferation and migration of GC cells via the CDCA8/p53 pathway.

关 键 词：蛋白超家族18B 胃癌 CDCA8/p53信号通路 细胞增殖 迁移 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]