噬菌体鸡尾酒制剂对牛奶中产志贺毒素大肠杆菌O157:H7的抑制作用  

Inhibitory Effect of Phage Cocktail Preparation on Shiga Toxin Producing E. coli O157:H7 in Milk

作　　者：季慕寅[1,2] 李敏 张海燕[2] 张爽[2] 马勋[3] 张炜[1] JI Muyin;LI Min;ZHANG Haiyan;ZHANG Shuang;MA Xun;ZHANG Wei(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;Wuhu Institute of Technology,Wuhu 241003,China;College of Animal Science and Technology,Shihezi University,Shihezi 832003,China)

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]芜湖职业技术学院,安徽芜湖241003 [3]石河子大学动物科技学院,新疆石河子832003

出　　处：《安徽科技学院学报》2021年第5期7-11,共5页Journal of Anhui Science and Technology University

基　　金：安徽省高校优秀中青年骨干人才国内外访学研修重点项目(gxfxZD2016358);芜湖职业技术学院校级科技团队(wzykjtd202002);国家自然科学基金新疆联合项目(U1803109)。

摘　　要：目的:研究噬菌体鸡尾酒混合制剂对牛奶中产志贺毒素大肠杆菌O157:H7的抑制作用研究。方法:采用双层平板法,以产志贺毒素大肠杆菌O157:H7 ATCC 43895为宿主菌,从污水中分离裂解性噬菌体。通过体外抑菌试验研究其在牛奶样品中的抑菌效果。结果:噬菌体C13.6、C13.9和C34.6的噬菌斑透亮清晰;能裂解宿主菌ATCC 43895及其他O157:H7大肠杆菌,为宽宿主谱;噬菌体鸡尾酒混合制剂对牛奶中O157:H7大肠杆菌的抑菌效果为:在25℃条件下,MOI=10,处理2 h及以上抑菌效果极其显著,在10 h时宿主菌数量下降4.30 (lg(CFU/mL))。结论:该噬菌体混合制剂对牛奶中O157:H7大肠杆菌有良好的抑制作用,具有作为食品防腐剂的潜能。Objective: To investigate the inhibitory effect of phage cocktail mixture on Shiga toxin producing E. coli O157:H7 in milk samples. Methods: The two-layer plate method was used to isolate the lytic phage from sewage with Shiga toxin producing Escherichia coli O157:H7 ATCC 43895 as the host bacteria. In addition, the antibacterial effect in milk samples was studied by antibacterial test in vitro. Results: The plaques of phages C13.6, C13.9 and C34.6 were clear. It can cleave the host bacteria ATCC 43895 and other O157:H7 Escherichia coli, which is a wide host spectrum;The bacteriostatic effect of phage cocktail mixture on E.coli O157:H7 in milk was as follows: at 25 ℃, MOI=10, the bacteriostatic effect was extremely significant after treatment for 2 h or more, and the number of host bacteria decreased by 4.30(lg(CFU/mL)) after treatment for 10 h. Conclusion: The phage cocktail had a good inhibitory effect on E. coli O157:H7 in milk, and had the potential as a food preservative.

关 键 词：O157:H7大肠杆菌 噬菌体鸡尾酒制剂 抑菌效果 牛奶 

分 类 号：S855.3[农业科学—临床兽医学]