基于核酸的纸基荧光生物传感器的设计及应用  

Design and Applications of Fluorogenic Nucleic Acid-Based Paper Biosensors

作　　者：杨爽 杨贤鹏 王宝俊 王蕾 Shuang Yang;Xianpeng Yang;Baojun Wang;Lei Wang(College of Environmental and Resource Sciences,Zhejiang University,Hangzhou 310058,China;Key Laboratory of Coastal Environment and Resources of Zhejiang Province(KLaCER),School of Engineering,Westlake University,Hangzhou 310024,China;College of Chemical and Biological Engineering,Zhejiang University,Hangzhou 310027,China;Hangzhou Innovation Center,Zhejiang University,Hangzhou 311200,China;School of Biological Sciences,University of Edinburgh,Edinburgh EH93FF,United Kingdom)

机构地区：[1]浙江大学环境与资源学院,杭州310058 [2]西湖大学工学院浙江省海岸带环境与资源研究重点实验室,杭州310024 [3]浙江大学化学工程与生物工程学院,杭州310027 [4]浙江大学杭州国际科创中心,杭州311200 [5]爱丁堡大学生物科学学院,爱丁堡EH93FF

出　　处：《化学进展》2021年第12期2309-2315,共7页Progress in Chemistry

基　　金：西湖大学科研启动经费(No.103256021901)资助。

摘　　要：纸基生物传感器由于其具有成本低、操作方便、生物可降解、识别元件用量低等优点,近年来受到了广泛的关注。其中,以功能核酸作为识别元件的纸基荧光生物传感器具有较高的灵敏度、瞬时响应以及实时检测等特性,在便携式传感设备方面展现出巨大的潜力。此外,将核酸作为识别元件的纸基无细胞蛋白合成平台,通过条件合成的报告荧光蛋白可实现对病毒、重金属等目标物的特异性检测,具有良好的应用前景。首先,本文介绍了基于核酸的纸基荧光生物传感器的设计,特别是基于核酸的识别元件与纸基材料的结合方式。其次,总结了基于核酸的纸基荧光生物传感器在临床诊断、食品安全检测、环境污染物检测等不同领域的最新研究进展,讨论了其优势与局限性。最后,探讨了基于核酸的纸基荧光生物传感器的发展方向与应用前景,以期为相关领域的研究提供参考。In recent years,paper-based biosensors have attracted increasing attention due to their low cost,ease of operation and disposal,biodegradability and low consumption of analytes.Among them,the paper-based fluorescent biosensors with functional nucleic acids as the recognition elements are of particular attraction.Their high sensitivity,instant response and real-time detection capabilities endow them with great potentials for applications in portable sensor devices.In addition,the paper-based cell-free protein synthesis platform,using nucleic acid as the recognition elements,can achieve specific detection of viruses,heavy metals and other targets by expressing the fluorescent proteins as the output reporter,which has good application prospects.Here we introduce the design of these fluorogenic nucleic acid-based paper biosensors,focusing on the integration methods of nucleic acid-based recognition elements and paper-based substrates.We also discuss the latest progress of their applications in different fields including clinical diagnosis,food contaminant detection and environmental pollutant detection as well as their advantages and limitations.Finally,the prospects and development directions of fluorogenic nucleic acid-based paper biosensors are presented,providing reference for research in related fields.

关 键 词：核酸 功能核酸 纸基材料 荧光响应 生物传感 

分 类 号：O657.3[理学—分析化学] TB381[理学—化学]