海藻酸盐水凝胶作为递送载体在组织再生中的应用  

Application of alginate hydrogel as a delivery vehicle in tissue regeneration

作　　者：臧帆 秦松[2,3] 王蕾[2] 王宇 李文军 林剑[1] Fan Zang;Song Qin;Lei Wang;Yu Wang;Wenjun Li;Jian Lin(College of Life Sciences,Yantai University,Yantai 264005,China;Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China;Center for Ocean Mega-Science,Chinese Academy of Sciences,Qingdao 266071,China)

机构地区：[1]烟台大学生命科学学院,烟台264005 [2]中国科学院烟台海岸带研究所,烟台264003 [3]中国科学院海洋大科学研究中心,青岛266071

出　　处：《中国科学：化学》2022年第1期23-37,共15页SCIENTIA SINICA Chimica

基　　金：山东省重大科技创新工程项目(编号:2019JZZY011103)资助。

摘　　要：海藻酸盐是一类存在于褐藻中的线性亲水多糖,由D-甘露糖醛酸(M)和L-古洛糖醛酸(G)以不同比例的重复单元组成.它是用于水凝胶合成的天然生物材料之一,通过简单的离子交联,即可与Ca2+等多价无机阳离子发生"蛋盒反应",形成水凝胶.海藻酸盐骨架上存在大量–OH和–COOH极性基团,通过化学或物理方法对其进行修饰,使其可以在温度、pH、光等刺激的响应下实现细胞或生物活性分子的可控释放.目前组织再生领域的主要应用策略之一是利用生物相容性材料,结合生物活性分子和细胞,以促进受损组织的再生.水凝胶材料在保护嵌入的细胞并模仿天然细胞外基质方面具有潜力.海藻酸盐也因为其易于凝胶化和良好的生物相容性,被广泛用于组织再生领域.本综述中,我们总结了用于组织再生,特别在伤口愈合、骨和心脏修复领域的海藻酸盐水凝胶的不同交联方法,重点分析了对刺激具有响应性的海藻酸盐水凝胶的特征以及其作为递送载体在组织再生中的应用.Alginate is a class of linear hydrophilic polysaccharides found in brown algae. It is composed of repeating units of D-mannuronic acid(M) and L-guluronic acid(G) in different proportions. It is one of the natural biological materials used for hydrogel synthesis. Through simple ion cross-linking, it can react with Ca2+and other multivalent inorganic cations to form hydrogel, which called “egg box reaction”. There are a large number of –OH and –COOH polar groups on the alginate skeleton, which can be modified by chemical or physical methods to achieve controlled release of cells or biologically active molecules in response to temperature, pH, light and other stimuli. At present, one of the main application strategies in the field of tissue regeneration is to use biocompatible materials, combined with bioactive molecules and cells, to promote the regeneration of damaged tissues. Hydrogel materials have the potential to protect embedded cells and mimic the natural extracellular matrix. Alginate is also widely used in the field of tissue regeneration due to its easy gelation and good biocompatibility. In this review, we summarized the different crosslinking methods of alginate hydrogels, focusing on the characteristics of alginate hydrogels that are responsive to stimuli and their applications as delivery vehicles in tissue regeneration.

关 键 词：海藻酸盐 水凝胶 共价和非共价交联 刺激响应性 递送载体 组织再生 

分 类 号：R318.08[医药卫生—生物医学工程] TQ427.26[医药卫生—基础医学]