六株冬虫夏草相关菌对蝙蝠蛾幼虫侵染特性的研究  

Relative infectiveness of six Cordyceps sinensis-related fungal strains to Hepialus armoricanus larvae

作　　者：谢放[1] 陈照禾 朱玉兰[1] 苏强军 陈晨[1] 张育[1] XIE Fang;CHEN Zhao-He;ZHU Yu-Lan;SU Qiang-Jun;CHEN Chen;ZHANG Yu(School of Biological and Pharmaceutical Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China)

机构地区：[1]兰州交通大学生物与制药工程学院,兰州730070

出　　处：《应用昆虫学报》2021年第6期1312-1321,共10页Chinese Journal of Applied Entomology

基　　金：国家自然科学基金项目(31560003)。

摘　　要：【目的】本文将冬虫夏草相关菌定义为分离自野生冬虫夏草Cordyceps sinensis,可以反接侵染蝙蝠蛾Hepialus armoricanus幼虫致死及与冬虫夏草菌Ophicordyceps sinensis对峙有相互影响的菌株。本文旨在对分离自冬虫夏草不同部位的6株相关菌单独或与冬虫夏草菌协同侵染蝙蝠蛾幼虫的效应进行研究。【方法】对6株相关菌进行生物学特征描述,并通过ITS序列比对进行种属鉴定;对6株相关菌做了与冬虫夏草菌的对峙实验及单独侵染蝙蝠蛾幼虫的实验;对其中的2株菌做了与冬虫夏草菌复合侵染蝙蝠蛾幼虫实验。【结果】证明了6株相关菌能够影响冬虫夏草菌的生长,其中菌株2F-2、FD、TB-1和TB-4对冬虫夏草菌抑制率接近100%,菌株3-5-2对冬虫夏草菌抑制率为4.32%;菌株MR-1对冬虫夏草菌抑制率为77.27%;6株相关菌均有侵染蝙蝠蛾幼虫的能力:菌株3-5-2对蝙蝠蛾幼虫侵染率为50.0%,菌株2F-2对蝙蝠蛾幼虫侵染率为85.0%,菌株MR-1对蝙蝠蛾幼虫侵染率为73.3%,菌株FD对蝙蝠蛾幼虫侵染率为70.0%,菌株TB-1对蝙蝠蛾幼虫侵染率为64.0%,菌株TB-4为65.0%;相对于单独侵染,复合侵染致死率有所下降。【结论】供试的6株相关菌均有侵染力并与冬虫夏草菌相互影响。[Objectives] To study the relative infectiousness of six fungal strains isolated from different parts of Cordyceps sinensis to Hepialus armoricanus larvae. C. sinensis-related fungi are defined as fungi isolated from wild C. sinensis that can infect and kill H. armoricanus larvae and interact with O. sinensis. [Methods] Biological characteristics of 6 strains of related fungi were described and their ITS sequences were aligned to identify the species. The infectiveness of the six strains of related fungi to H. armoricanus larvae were tested separately. Two related fungi were tested in a joint infection experiment with O. sinensis. [Results] The 6 strains of related fungi all affected the growth of O. sinensis: The inhibition rate of the 2 F-2,FD, TB-1 and TB-4 strains to O. sinensis was close to 100%, that of the 3-5-2 strain was 4.32% and that of the MR-1 strain was 77.27%. All six strains had the ability to infect H. armoricanus larvae. The infection rate of the 3-5-2 strain was 50.0%,that of the 2 F-2 strain was 85.0% and that of the MR-1 strain was 73.3%. The infection rate of FD strain was 70.0%;the infection rate of the TB-1 strain was 64.0% and the infection rate of the TB-4 strain was 65.0%. The mortality rate of joint infections was lower than that of single-strain infections. [Conclusion] The 6 strains of related fungi were all infectious to H.armoricanus larvae and can interact with O. sinensis.

关 键 词：冬虫夏草相关菌 蝙蝠蛾幼虫 冬虫夏草菌 侵染 

分 类 号：S567.35[农业科学—中草药栽培]