南美斑潜蝇的快速冷耐受及其生理生化效应  

Physiological and biochemical effects of rapid cold hardening in Liriomyza huidobrensis

作　　者：张君怡 张小香 宋英[3] 常亚文[1] 杜予州[1,2] ZHANG Jun-Yi;ZHANG Xiao-Xiang;SONGYing;CHANG Ya-Wen;DU Yu-Zhou(College of Horticulture and Plant Protection,Institute of Applied Entomology,Yangzhou University,Yangzhou 225009,China;Joint Laboratory for International Cooperation in Agriculture and Agricultural Product Safety,Ministry of Education,Yangzhou 225009,China;Taihu Agricultural Research Institute of Jiangsu,Suzhou 215155,China)

机构地区：[1]扬州大学园艺与植物保护学院暨应用昆虫研究所,扬州225009 [2]教育部农业与农产品安全国际合作联合实验室,扬州225009 [3]江苏太湖地区农业科学研究所,苏州215155

出　　处：《应用昆虫学报》2021年第6期1376-1384,共9页Chinese Journal of Applied Entomology

基　　金：江苏现代农业(西甜瓜)产业技术体系绿色防控创新团队(JATS[2020]309);大学生创新训练项目(X20190581)。

摘　　要：【目的】为明确南美斑潜蝇Liriomyza huidobrensis经快速冷耐受(Rapid cold hardening,RCH)处理后的基础耐寒性的变化,以探究南美斑潜蝇RCH的生理生化机制。【方法】根据初步筛选的胁迫温度和驯化时间的结果,将南美斑潜蝇蛹直接进行低温胁迫(Coldshock,CS)和先进行RCH驯化后再进行低温胁迫(RCH+CS)后,记录南美斑潜蝇蛹的羽化率;利用热电偶方法测定驯化后和未驯化蛹的过冷却点;分别采用气相色谱-质谱仪联用法(GC-MS)、液相色谱-质谱联用技术(HPLC-MS)及分光光度计测定不同处理模式下南美斑潜蝇的脂肪酸、糖类以及甘油的含量。【结果】经RCH驯化后再进行低温胁迫的南美斑潜蝇蛹的羽化率显著增加(由20.00%增至45.56%,F_(3.8)=10.37,P<0.05),但过冷却点(SCP)却没有明显变化(未驯化-19.90℃,驯化后-20.10℃,t=0.703,P>0.05);RCH驯化后南美斑潜蝇体内棕榈油酸含量增加(由5.96%增至19.26%,F_(3.8)=52.717,P<0.05),棕榈酸含量下降(由24.32%降至2.94%,F_(3.8)=65.456,P<0.05),总不饱和脂肪酸和饱和脂肪酸比值升高(由2.17增至10.94,F_(3.8)=64.347,P<0.05);RCH驯化后南美斑潜蝇体内的海藻糖(由5.46μg/mg增至7.71μg/mg,F_(3.8)=9.912,P<0.05)和甘油的含量升高(由1.177μg/mg增至1.314μg/mg,F_(3.8)=6.428,P<0.05),而葡萄糖含量下降(由1.84μg/mg降至1.22μg/mg,F_(3.8)=191.298,P<0.05)。【结论】南美斑潜蝇RCH与脂肪酸的不饱和度增加以及海藻糖、葡萄糖和甘油这3种抗冻物质均有关。[Objectives] To determine changes in the basic cold tolerance of Liriomyza huidobrensis after undergoing rapid cold hardening(RCH), and to investigate the physiological and biochemical effects of RCH in this species. [Methods]According to the results of a pilot study of temperature stress and acclimation time, the emergence rate of L. huidobrensis pupae was recorded after RCH acclimation and subsequently under low temperature stress(CS). A thermocouple was used to determine the supercooling point of acclimated and unacclimated pupae. GC-MS, HPLC-MS and spectrophotometer were used to determine the amount of fatty acids, sugars and glycerol in pupae under different temperature treatments. [Results] The emergence rate of L. huidobrensis pupae increased significantly(20.00% to 45.60%)(F_(3.8)=10.37, P<0.05) after RCH acclimation and subsequent exposure to low temperature stress but their supercooling point(SCP) did not change significantly(unacclimated: -19.90 ℃, after acclimation: -20.10 ℃)(t=0.703, P>0.05). After RCH, the content of palmitoleic acid increased(from 5.96% to 19.26%)(F_(3.8)=52.717, P<0.05) whereas the content of palmitic acid decreased(from 24.32% to 2.94%)(F_(3.8)=65.456, P<0.05). The ratio of total unsaturated fatty acids to saturated fatty acids increased(from 2.17 to 10.94)(F_(3.8)=64.347, P<0.05). After acclimation, trehalose content increased(5.46 μg/mg to 7.71 μg/mg)(F_(3.8)=9.912, P<0.05),glycerol content increased(from 1.177 μg/mg to 1.314 μg/mg)(F_(3.8)=6.428, P<0.05) and glucose content decreased(from 1.84μg/mg to 1.22 μg/mg)(F_(3.8)=191.298, P<0.05). [Conclusion] RCH in L. huidobrensis causes an increase in unsaturated fatty acids and cryoprotectants, including trehalose, glucose and glycerin.

关 键 词：南美斑潜蝇 快速冷耐受 耐寒性 脂肪酸 抗冻物质 

分 类 号：S433[农业科学—农业昆虫与害虫防治]