昆虫病原线虫侵染大蜡螟的行为观测  

Infection of Galleria mellonella by the entomopathogenic nematodes Heterorhabditis megidis 0627M and Steinernema felitiae 0619HT

作　　者：吴霞 李小玲 钱秀娟 WU Xia;LI Xiao-Ling;QIAN Xiu-Juan(Plant Protection Academy,Gansu Agricultural University,Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province,Lanzhou 730070,China;Plant Protection Academy,Southwest University,Chongqing 400700,China)

机构地区：[1]甘肃农业大学植物保护学院,甘肃省农作物病虫害生物防治工程实验室,兰州730070 [2]西南大学植物保护学院,重庆400700

出　　处：《应用昆虫学报》2021年第6期1417-1425,共9页Chinese Journal of Applied Entomology

基　　金：国家自然科学基金(31960559);甘肃农业大学科技创新基金-学科建设专项(GAU-XKJS-151);甘肃省科技计划项目(21JR7RA819);甘肃省教育厅优秀研究生“创新之星”项目(2021CXZX-410)。

摘　　要：【目的】为探明昆虫病原线虫在感染寄主过程中的搜寻和识别行为,以及对寄主昆虫的趋向行为和迁移能力。【方法】开展了室内生测试验,观察异小杆线虫Heterorhabditis megidis 0627M和斯式线虫Steinernema felitiae 0619HT感染寄主大蜡螟Galleria mellonella的整个过程,包括2种线虫对寄主的搜寻和识别,以及侵入整个过程,同时观测线虫对寄主的趋向行为和迁移行为特征。【结果】异小杆线虫和斯氏线虫均对大蜡螟表现出明显的趋性,且水分和昆虫病原线虫的搜寻寄主的方式影响感染期线虫对寄主的侵染过程;2种线虫本身迁移能力较强,大蜡螟的存在对其迁移能力有影响。【结论】2种线虫相比较而言,当有大蜡螟存在时,异小杆线虫比斯式线虫表现出更强的趋性,且寄主存在对线虫迁移能力的影响,斯式线虫大于异小杆线虫。[Objectives] To investigate the infection of Galleria mellonella by the entomopathogenic nematodes Heterorhabditis megidis 0627 M and Steinernema felitiae 0619 HT. [Methods] We investigated the behavior of H. megidis 0627 M and S. felitiae 0619 HT’s with, and without, G. mellonella, including host searching and recognition and the entire infestation process. We also investigated the movement of nematodes towards host insects. [Results] The presence of water, and the method of searching for hosts, affected the infection process. Both H. megidis 0627 M and S. felitiae 0619 HT showed obvious tropism towards G. mellonella. [Conclusion] H. megidis 0627 M had greater mobility than S. felitiae 0619 HT in the presence of G. mellonella, however, in the absence of G. mellonella, S. felitiae 0619 HT’s was more mobile than H. megidis 0627 M.

关 键 词：昆虫病原线虫 线虫行为 感染过程 趋向行为 迁移能力 

分 类 号：S476.15[农业科学—农业昆虫与害虫防治]