银耳栽培菌株的线粒体内含子多态性分析  

Polymorphism of Mitochondrial Introns Among Tremella fuciformis Cultivars

作　　者：林隆基 黄海辰 钟馨林 李岳峰 钟长科 谢宝贵[1] 明瑞光[4] 邓优锦[1] LIN Longji;HUANG Haichen;ZHONG Xinlin;LI Yuefeng;ZHONG Changke;XIE Baogui;MING Ray;DENG Youjin(College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;College of Forestry,Fujian agriculture and forestry university,Fuzhou 350002,Fujian,China;Gutian County Agricultural Bureau,Ningde 352229,Fujian,China;Center for Genomics and Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China)

机构地区：[1]福建农林大学生命科学学院,福建福州350002 [2]福建农林大学林学院,福建福州350002 [3]古田县农业局,福建宁德352229 [4]福建农林大学基因组与生物技术研究中心,福建福州350002

出　　处：《食用菌学报》2022年第1期1-11,共11页Acta Edulis Fungi

基　　金：福建省中央引导地方科技发展资金(2021L3006);国家自然科学基金(31670021)。

摘　　要：为从线粒体角度了解银耳(Tremella fuciformis)主要栽培菌株的遗传多样性,从37个银耳栽培菌株中选取33、34和37号3个典型菌株进行高通量基因组测序,并进行线粒体基因组组装和分析,结果表明:3个菌株的线粒体基因组序列一致,但就线粒体基因组拷贝数而言,33号显著高于34号、37号。选取37号菌株线粒体基因组与已公布的7个野生菌株进行内含子多态性分析,发现它们内含子数量和插入位点各不相同,存在丰富的多态性。针对37号菌株特异内含子及周边序列设计8对引物,通过PCR扩增分析栽培菌株的多样性,结果表明:这8对引物在36个栽培菌株中均扩增出一条长度一致的条带,其长度与37号菌株的预测长度相同,说明36个菌株均含有与37号菌株相同的内含子。Mitochondrial DNA diversity of major cultivated strains of Tremella fuciformis was studied.Using high-throughput sequencing,three certificated cultivars of T.fuciformis,strain No.33,No.34 and No.37,were subjected to whole genome sequencing.Then their mitochondrial genome sequences were assembled and analyzed.The results showed that strain No.33,34 and 37 were identical in mitochondrial genome sequence,but the DNA copy number of strain No.33 was greater than that of strain No.34 and No.37.Analysis of mitochondrial intron polymorphism in strain No.37 and seven previously published wild type strains showed a rich diversity in number of introns and insertion positions between these strains.Using eight pairs of primers specific to the mitochondrial genome of strain No.37,PCR analyses were carried out on 36 cultivated strains.For all the primers,there was a band with the same length as predicted in strain No.37 in all 36 strains,suggesting all 36 strains contained the same intron as strain No.37.

关 键 词：银耳 线粒体基因组 内含子 多态性分析 

分 类 号：S567.34[农业科学—中草药栽培]